Document Detail

Interactions between fatty acids and arginine metabolism: implications for the design of immune-enhancing diets.
MedLine Citation:
PMID:  15709549     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Trauma increases the enzyme arginase, thus depleting arginine necessary for producing nitric oxide. Arginine and omega-3 fatty acids are components in immune-enhancing diets. These diets decrease infections in surgical patients, perhaps by preventing arginine deficiency. This study examines whether omega-3 fatty acids alter the metabolic fate of arginine. Thus, we hypothesized there could be differential effects of varying prostaglandins on regulation of arginase. METHODS: Prostaglandins PGE1, PGE2, and PGE3 were tested using RAW 264.7 cells cultured in the presence of these prostaglandins for 24 hours. IL-13 (10 ng/mL) was added 24 hours later to induce arginase I. NO production was induced by adding LPS (2 microg/mL) to the cultures after another 24 hours. RESULTS: Arginase activity (nmol/min/mg) was induced by all prostaglandins but significantly more by PGE1 (466.05+/-30.25) and PGE2 (248.45+/-15.05) than PGE3 (139.87+/-19.88; p < .002) when co-cultured with IL-13. Western blots correlated the increase in arginase I expression. Nitrate levels (microM) were inversely proportional to activity with PGE3 having the highest production (3.89+/-0.19) and PGE2 and PGE1 with the lowest (2.75+/-0.49 and 1.54+/-0.19, respectively). Inhibition of arginase I using nor-hydroxyarginine increased and equalized nitrate levels. CONCLUSIONS: Different prostaglandins significantly alter the metabolism of arginine. Prostaglandins from omega-6 fatty acids increases arginase I expression. By decreasing arginase I expression, prostaglandins from omega-3 fatty acids may increase available arginine. The specific combinations of dietary fatty acids and arginine should be considered when tailoring dietary regimens.
Vishal Bansal; Kimberly M Syres; Valeryia Makarenkova; Ryan Brannon; Benjamin Matta; Brian G Harbrecht; Juan B Ochoa
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  JPEN. Journal of parenteral and enteral nutrition     Volume:  29     ISSN:  0148-6071     ISO Abbreviation:  JPEN J Parenter Enteral Nutr     Publication Date:    2005 Jan-Feb
Date Detail:
Created Date:  2005-02-15     Completed Date:  2005-07-07     Revised Date:  2007-02-21    
Medline Journal Info:
Nlm Unique ID:  7804134     Medline TA:  JPEN J Parenter Enteral Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  S75-80     Citation Subset:  IM    
Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA.
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MeSH Terms
Arginase / metabolism*
Arginine / deficiency,  metabolism*
Fatty Acids / metabolism*
Fatty Acids, Omega-3 / metabolism
Fatty Acids, Omega-6 / metabolism
Gene Expression Regulation / drug effects
Nitric Oxide / metabolism
Prostaglandins / administration & dosage*,  pharmacology
Reg. No./Substance:
0/Fatty Acids; 0/Fatty Acids, Omega-3; 0/Fatty Acids, Omega-6; 0/Prostaglandins; 10102-43-9/Nitric Oxide; 74-79-3/Arginine; EC

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