| Interactions between Swi1-Swi3, Mrc1 and S phase kinase, Hsk1 may regulate cellular responses to stalled replication forks in fission yeast. | |
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MedLine Citation:
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PMID: 19422421 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The Swi1-Swi3 replication fork protection complex and Mrc1 protein are required for stabilization of stalled replication forks in fission yeast. Hsk1 kinase also plays roles in checkpoint responses elicited by arrested replication forks. We show that both Swi1 and Swi3, the abundance of which are interdependent, are required for chromatin association of Mrc1. Co-immunoprecipitation experiments show the interactions of Swi1-Swi3, Mrc1 and Hsk1. Mrc1 interacts with Swi3 and Hsk1 proteins through its central segment (378-879) containing a SQ/TQ cluster, and this segment is sufficient for checkpoint reaction. The SQ/TQ cluster segment (536-673) is essential but not sufficient for the interactions and for resistance to replication inhibitor hydroxyurea. Mrc1 protein level is increased in hsk1-89 cells due to apparent stabilization, and we have identified a potential phosphodegron sequence. These results suggest that interactions of the Swi1-Swi3 complex and Hsk1 kinase with Mrc1 may play a role in cellular responses to stalled replication forks in fission yeast. |
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Authors:
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Michie Shimmoto; Seiji Matsumoto; Yukari Odagiri; Eishi Noguchi; Paul Russell; Hisao Masai |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-04-30 |
Journal Detail:
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Title: Genes to cells : devoted to molecular & cellular mechanisms Volume: 14 ISSN: 1365-2443 ISO Abbreviation: Genes Cells Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-09-18 Completed Date: 2009-12-11 Revised Date: 2013-01-30 |
Medline Journal Info:
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Nlm Unique ID: 9607379 Medline TA: Genes Cells Country: England |
Other Details:
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Languages: eng Pagination: 669-82 Citation Subset: IM |
Affiliation:
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Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 113-8613, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle Proteins
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genetics,
metabolism* Chromatin / metabolism DNA-Binding Proteins / genetics, metabolism* Gene Expression Regulation, Fungal Immunoprecipitation Protein-Serine-Threonine Kinases / genetics, metabolism* S Phase / physiology* Schizosaccharomyces* / genetics, metabolism, physiology Schizosaccharomyces pombe Proteins / genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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GM077604/GM/NIGMS NIH HHS; R01 GM077604/GM/NIGMS NIH HHS; R01 GM077604-01A2/GM/NIGMS NIH HHS; R01 GM077604-02/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/Chromatin; 0/DNA-Binding Proteins; 0/MRC1 protein, S pombe; 0/Schizosaccharomyces pombe Proteins; 0/Swi3 protein, S pombe; 0/swi1 protein, S pombe; EC 2.7.11.1/HSK1 protein, S pombe; EC 2.7.11.1/Protein-Serine-Threonine Kinases |
| Comments/Corrections | |
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