Document Detail

Interactions between LIM domains and the LIM domain-binding protein Ldb1.
MedLine Citation:
PMID:  9468533     Owner:  NLM     Status:  MEDLINE    
LIM domains mediate protein-protein interactions and, within LIM-homeodomain proteins, act as negative regulators of the transcriptional activation function of the protein. The recently described protein Ldb1 (also known as NLI; LIM domain-binding protein) binds LIM domains in vitro and synergizes with the LIM-homeodomain protein Xlim-1 in frog embryo microinjection experiments. In this study we localized the transcriptional activation domain of Xlim-1 to its carboxyl-terminal region, and characterized the interactions of the amino-terminally located LIM domains with Ldb1. Ldb1 binds LIM domains through its carboxyl-terminal region, and can form homodimers via its amino-terminal region. Optimal binding to Ldb1 required tandem LIM domains, while single domains could bind at lower but clearly measurable efficiency. In animal explant experiments, synergism of Ldb1 with Xlim-1 in the activation of downstream genes required both the region containing the dimerization domain of Ldb1 and the region containing the LIM-binding domain. The role of Ldb1 may be to recruit other transcriptional activators depending on the promoter context and LIM-homeodomain partner involved.
J J Breen; A D Agulnick; H Westphal; I B Dawid
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  273     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-03-19     Completed Date:  1998-03-19     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4712-7     Citation Subset:  IM    
Laboratory of Molecular Genetics, NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA.
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MeSH Terms
DNA-Binding Proteins / metabolism*
Homeodomain Proteins / metabolism*
Protein Binding
Recombinant Proteins / metabolism
Transcriptional Activation
Xenopus Proteins*
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Homeodomain Proteins; 0/Lim-1 protein, Xenopus; 0/Recombinant Proteins; 0/XLdb1 protein, Xenopus; 0/Xenopus Proteins

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