| Interactions between CD36 and global intestinal alkaline phosphatase in mouse small intestine and effects of high-fat diet. | |
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MedLine Citation:
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PMID: 21900644 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The mechanisms of the saturable component of long chain fatty acid (LCFA) transport across the small intestinal epithelium, and how it is regulated by consumption of a high-fat diet (HFD), are uncertain. It is hypothesized here that the putative fatty acid translocase/CD36 and intestinal alkaline phosphatases (IAPs) function together to optimize LCFA transport. Phosphorylated CD36 (pCD36) was expressed in mouse enterocytes, and could be dephosphorylated by calf intestinal alkaline phosphatase (CIAP). Uptake of a fluorescently-tagged LCFA analog into isolated enteroctyes was increased when cells were treated with CIAP; this was blocked with a specific CD36 inhibitor. pCD36 co-localized in enterocytes with the global IAP (gIAP) isozyme, and specifically co-immunoprecipitated with gIAP but not the duodenal-specific isozyme (dIAP). Purified recombinant gIAP dephosphorylated pCD36. Body weight, adiposity, plasma leptin and triglycerides were significantly increased in HFD mice compared to normal-fat diet controls. HFD significantly increased immunoreactive CD36 and gIAP but not dIAP, in jejunum but not duodenum. Uptake of the LCFA analog was increased in a CD36-dependent manner in enterocytes from HFD mice. It is concluded that CD36 exists in its phosphorylated and dephosphorylated states in mouse enterocytes, that pCD36 is a specific substrate of gIAP, and that dephosphorylation by IAPs results in increased LCFA transport capability. HFD upregulates CD36 and gIAP proteins in parallel, and enhances CD36-dependent fatty acid uptake. The interactions between these proteins may be important for efficient fat transport in mouse intestine, but whether the observed changes in gIAP and CD36 in enterocytes are contributing factors to HFD-induced obesity remains to be determined. |
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Authors:
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Matthew D Lynes; Sonoko Narisawa; Jose Luis Millan; Eric P Widmaier |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-7 |
Journal Detail:
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Title: American journal of physiology. Regulatory, integrative and comparative physiology Volume: - ISSN: 1522-1490 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-9-8 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901230 Medline TA: Am J Physiol Regul Integr Comp Physiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1Boston University. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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