Document Detail

Interactions of aromatic residues of proteins with nucleic acids. Fluorescence studies of the binding of oligopeptides containing tryptophan and tyrosine residues to polynucleotides.
MedLine Citation:
PMID:  234245     Owner:  NLM     Status:  MEDLINE    
The binding of oligopeptides of general structure Lys-X-Lys (where X is an aromatic residue) to several polynucleotides has been studied by fluorescence spectroscopy. Two types of complexes are formed, both involving electrostatic interactions between lysyl residues and phosphate groups as shown by the ionic strength and pH dependence of binding. The fluorescence quantum yield of the first complex is identical with that of the free peptide. The other complex involves a stacking of the nucleic acid bases with the aromatic amino acid whose fluorescence is quenched. Fluorescence data have been quantitatively analyzed according to a model involving these two types of complexes. Association constants and the size of binding sites have been determined. Stacking interactions are favored in single-stranded polynucleotides as compared to double-stranded ones. A short oligopeptide such as Lys-X-Lys is thus able to distinguish between single-stranded and double-stranded nucleic acids. Fluorescence results are compared to those obtained by proton magnetic resonance and circular dichroism.
F Brun; J J Toulmé; C Hélène
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Biochemistry     Volume:  14     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1975 Feb 
Date Detail:
Created Date:  1975-05-05     Completed Date:  1975-05-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  558-63     Citation Subset:  IM    
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MeSH Terms
Binding Sites
Hydrogen-Ion Concentration
Nucleic Acid Conformation
Organophosphorus Compounds
Osmolar Concentration
Spectrometry, Fluorescence
Reg. No./Substance:
0/Oligopeptides; 0/Organophosphorus Compounds; 0/Polynucleotides; 55520-40-6/Tyrosine; 56-87-1/Lysine; 73-22-3/Tryptophan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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