| Interactions of acridine orange with nucleic acids. Properties of complexes of acridine orange with single stranded ribonucleic acid. | |
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MedLine Citation:
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PMID: 6197975 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Interactions between acridine orange (AO) and nucleic acids (calf thymus DNA, and homoribo- and homodeoxyribo-polynucleotides) were studied in solutions containing ethanol as a cosolvent. Light absorption, scattering and luminescence were measured as a function of AO concentration at different dye/phosphate (D/P) ratios, and the data were analyzed using the McGhee-von Hippel probabilistic model of the polymer-ligand interactions. The absorption spectra of AO complexes with four homoribopolymers are presented. The intrinsic association constants and cooperativity coefficients of the formation of the complexes were calculated. The effects of ethanol (up to 35%, v/v) on these interactions were concentration dependent and may be extrapolated to zero concentration of this cosolvent. The possibility of destabilization of the double helix of nucleic acids by AO at high D/P ratios is discussed in light of the available thermodynamic data. |
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Authors:
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J Kapuscinski; Z Darzynkiewicz; M R Melamed |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemical pharmacology Volume: 32 ISSN: 0006-2952 ISO Abbreviation: Biochem. Pharmacol. Publication Date: 1983 Dec |
Date Detail:
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Created Date: 1984-02-14 Completed Date: 1984-02-14 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0101032 Medline TA: Biochem Pharmacol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 3679-94 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acridine Orange
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pharmacology* Animals Binding Sites / drug effects Cattle Chemical Phenomena Chemistry DNA / analysis* Ethanol Light Models, Chemical RNA / analysis* Scattering, Radiation Spectrometry, Fluorescence Spectrophotometry Thymus Gland / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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CA-14134/CA/NCI NIH HHS; CA-232-96/CA/NCI NIH HHS; CA-28704/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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63231-63-0/RNA; 64-17-5/Ethanol; 65-61-2/Acridine Orange; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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