Document Detail


Interaction with Ppil3 leads to the cytoplasmic localization of Apoptin in tumor cells.
MedLine Citation:
PMID:  18474220     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Apoptin, a small protein encoded by chicken anemia virus (CAV), induces cell death specifically in cancer cells. In normal cells, Apoptin remains in the cytoplasm; whereas in cancerous cells, it migrates into the nucleus and kills the cell. Cellular localization appears to be crucial. Through a yeast two-hybrid screen, we identified human Peptidyl-prolyl isomerase-like 3 (Ppil3) as one of the Apoptin-associated proteins. Ppil3 could bind Apoptin directly, and held Apoptin in cytoplasm even in tumor cells. We then demonstrated that the nuclearcytoplasmic distribution of Apoptin is related to the expression level of intrinsic Ppil3. Moreover, extrinsic modifying of Ppil3 levels also resulted in nuclearcytoplasmic shuffling of Apoptin. The Apoptin P109A mutant, located between the putative nuclear localization and export signals, could significantly impair the function of Ppil3. Our results suggest a new direction for the localization mechanism study of Apoptin in cells.
Authors:
De-Hua Huo; Li-Na Yi; Jine Yang
Publication Detail:
Type:  Journal Article     Date:  2008-05-12
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  372     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-06-09     Completed Date:  2008-06-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  14-8     Citation Subset:  IM    
Affiliation:
The Key Laboratory of Gene Engineering of Ministry of Education, School of Life Sciences, Sun Yat-Sen (Zhongshan) University, 135th Xin-Gang-Xi Road, Guangzhou 510275, PR China.
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MeSH Terms
Descriptor/Qualifier:
Active Transport, Cell Nucleus
Amino Acid Sequence
Capsid Proteins / genetics,  metabolism*
Cell Line, Tumor
Cell Nucleus / metabolism
Cyclophilins / analysis,  genetics,  metabolism*
Cytoplasm / enzymology,  metabolism*
Humans
Molecular Sequence Data
Mutation
Neoplasms / enzymology,  metabolism*
Two-Hybrid System Techniques
Chemical
Reg. No./Substance:
0/Capsid Proteins; 0/VP3 protein, Chicken anemia virus; EC 5.2.1.-/Cyclophilins; EC 5.2.1.-/cyclophilin J, human

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