Document Detail

Interaction of tetanus toxin and toxoid with cultured neuroblastoma cells. Analysis by immunofluorescence.
MedLine Citation:
PMID:  320489     Owner:  NLM     Status:  MEDLINE    
The primary interaction of tetanus toxin and toxoid with mouse neuroblastoma cells (C 1300, clone NB2A) in tissue culture was studied using direct immunofluorescence. Experiments were done in standard routine cultures and also those influenced by chemical modulators. There is a difference in the characteristic binding response between the growth culture cells (grown in presence of fetal calf serum) and differentiating culture cells (grown in absence of serum). Exposure to the toxin gives no visible effect on the cell division or viability in growth cultures; whereas in differentiating cells the processes are shortened and the adherence to the glass is diminished without involving significant cell death. The toxoid did not bind at all under the same experimental conditions. Since there was no biological effect in growth cultures we have called this binding ineffective, and in the case of the differentiating cells, effective binding. Stimulation of pinocytosis increases the uptake of toxin in both cultures. Presence of some surface bound toxin still remaining on the differentiating cells indicates the possibility of another sort of mechanism for internalization. Pre-treatment of the cells with neuraminidase or beta-galactosidase to alter the membrane gangliosides eliminates binding in growth cultures but not in differentiating cultures. From these results we suggest that even though the toxin may well bind to gangliosides, at least in the differentiating cultures they are not solely responsible for the fixation. The morphologically observed effective binding is probably that not related to gangliosides.
J M Zimmerman; J C Piffaretti
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Naunyn-Schmiedeberg's archives of pharmacology     Volume:  296     ISSN:  0028-1298     ISO Abbreviation:  Naunyn Schmiedebergs Arch. Pharmacol.     Publication Date:  1977 Feb 
Date Detail:
Created Date:  1977-04-25     Completed Date:  1977-04-25     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0326264     Medline TA:  Naunyn Schmiedebergs Arch Pharmacol     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  271-7     Citation Subset:  IM    
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MeSH Terms
Binding Sites
Cell Membrane / metabolism
Cell Survival / drug effects
Cells, Cultured
Fluorescent Antibody Technique
Gangliosides / metabolism
Neuroblastoma / metabolism*,  pathology,  ultrastructure
Tetanus Toxin / metabolism*,  pharmacology
Tetanus Toxoid / metabolism*,  pharmacology
Reg. No./Substance:
0/Gangliosides; 0/Tetanus Toxin; 0/Tetanus Toxoid

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