Document Detail

Interaction of sulfated glycosaminoglycans with lectins.
MedLine Citation:
PMID:  6787039     Owner:  NLM     Status:  MEDLINE    
The sulfated glycosaminoglycans, such as keratan sulfate and chitin sulfate having 3-hydroxy free N-acetyl-beta-D-glucosaminyl residues as constituents, reacted with wheat germ agglutinin and Solanum tuberosum agglutinin by sugar-specific interaction. The glycosaminoglycans showed different inhibitory activities to the hemagglutination reaction of these lectins and keratan sulfate and its modified products formed insoluble complexes with both of the lectins at pH 7.0 in physiological saline solutions (0.15 M NaCl). S. tuberosum agglutinin was precipitated within a particularly narrow concentration range of keratan sulfate, and the formation of a soluble complex was observed by gel chromatography. These interactions were specifically inhibited by N,N'-diacetylchitobiose but not by 2 M NaCl. The specific interactions of the glycosaminoglycans with S. tuberosum agglutinin were confirmed by their ultraviolet difference spectra with two peaks at 285 and 298 nm attributable to the tryptophan residues in the binding site of the agglutinin. It was also found that S. tuberosum agglutinin and wheat germ agglutinin have different binding specificities. The presence of sulfate groups in either keratan sulfate or chitin sulfate did not interfere with their specific interactions with S. tuberosum agglutinin as strongly as with wheat germ agglutinin. The N-acetylneuraminic acid residues in keratan sulfate were found to be receptor sites for wheat germ agglutinin but not for S. tuberosum agglutinin.
N Toda; A Doi; A Jimbo; I Matsumoto; N Seno
Related Documents :
2029579 - Antithrombin-iii-hamilton, ala 382 to thr: an antithrombin-iii variant that acts as a s...
21644529 - N-fused imidazoles as novel anticancer agents that inhibit catalytic activity of topois...
17875649 - Mechanism by which exosites promote the inhibition of blood coagulation proteases by he...
19706609 - Para-nitrophenyl sulfate activation of human sulfotransferase 1a1 is consistent with in...
18265159 - Analysis of sulfate esters by solvolysis or hydrolysis.
15140589 - A small fraction of dermatan sulfate with significantly increased anticoagulant activit...
17679139 - Crystal structure of cytochrome p450 moxa from nonomuraea recticatena (cyp105).
19666559 - Variations in the colchicine-binding domain provide insight into the structural switch ...
22713569 - The c-terminus of troponin t is essential for maintaining the inactive state of regulat...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  256     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1981 Jun 
Date Detail:
Created Date:  1981-08-10     Completed Date:  1981-08-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5345-9     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Chondroitin Sulfates
Hemagglutination Inhibition Tests
Heparitin Sulfate
Keratan Sulfate
Spectrophotometry, Ultraviolet
Structure-Activity Relationship
Sulfuric Acids
Wheat Germ Agglutinins
Reg. No./Substance:
0/Glycosaminoglycans; 0/Lectins; 0/Sulfuric Acids; 0/Wheat Germ Agglutinins; 9007-28-7/Chondroitin Sulfates; 9050-30-0/Heparitin Sulfate; 9056-36-4/Keratan Sulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Effect of various lipoxygenase metabolites of arachidonic acid on degranulation of polymorphonuclear...
Next Document:  An alpha-D-galactosyltransferase activity in Ehrlich ascites tumor cells. Biosynthesis and character...