Document Detail


Interaction of steroid--peroxidase conjugates with cellulose immunosorbents in aqueous and micellar media.
MedLine Citation:
PMID:  9864448     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In 0.1 M bicarbonate buffer (pH 9.0) and in microemulsions of aerosol OT (AOT) and its mixture with Triton X-45 in heptane, antibodies against cortisol (anti-COR) and progesterone (anti-PROG) were covalently immobilized on fine-porous cellulose filters (0.6 cm diameter) after sodium periodate oxidation. Immunosorbents obtained in different media were characterized in terms of antibody-bound density and antigen-binding capacity with respect to peroxidase--steroid conjugates HP--COR-11 and HP--PROG-4 containing 11 molecules of cortisol and four progesterone molecules, respectively. For all immunosorbents antigen-binding capacity expressed as peroxidase activity of immune complexes formed on the solid cellulose phase in aqueous and micellar media was determined. Dissociation constants of immunocomplexes on the cellulose formed in aqueous and micellar media were determined using ELISA. In all cases Kd values in aqueous media were approximately 10-8 M and were significantly lower than corresponding values of dissociation constants of immune complexes in mixed microemulsions of AOT and Triton X-45.
Authors:
A N Eryomin; D I Metelitza
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry. Biokhimii͡a     Volume:  63     ISSN:  0006-2979     ISO Abbreviation:  Biochemistry Mosc.     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1999-02-08     Completed Date:  1999-02-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376536     Medline TA:  Biochemistry (Mosc)     Country:  RUSSIA    
Other Details:
Languages:  eng     Pagination:  1148-59     Citation Subset:  IM    
Affiliation:
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk, 220141, Belarus. ibochbel@ns.igs.ac.by
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies / isolation & purification
Antigen-Antibody Complex / isolation & purification
Cellulose
Horseradish Peroxidase*
Hydrocortisone / immunology*
Immunosorbent Techniques*
Kinetics
Micelles
Progesterone / immunology*
Water
Chemical
Reg. No./Substance:
0/Antibodies; 0/Antigen-Antibody Complex; 0/Micelles; 50-23-7/Hydrocortisone; 57-83-0/Progesterone; 7732-18-5/Water; 9004-34-6/Cellulose; EC 1.11.1.-/Horseradish Peroxidase

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