| Interaction of the retinoic acid signaling pathway with spicule formation in the marine sponge Suberites domuncula through activation of bone morphogenetic protein-1. | |
| | |
MedLine Citation:
|
PMID: 21952113 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
BACKGROUND: The formation of the spicules in siliceous sponges involves the formation of cylinder-like structures in the extraspicular space, composed of the enzyme silicatein and the calcium-dependent lectin. SCOPE OF REVIEW: Molecular cloning of the cDNAs (carotene dioxygenase, retinal dehydrogenase, and BMB-1 [bone morphogenic protein-1]) from the demosponge Suberites domuncula was performed. These tools were used to understand the retinoid metabolism in the animal by qRT-PCR, immunoblotting and TEM. MAJOR CONCLUSIONS: We demonstrate that silintaphin-2, a silicatein-interacting protein, is processed from a longer-sized 15-kDa precursor to a truncated, shorter-sized 13kDa calcium-binding protein via proteolytic cleavage at the dipeptide Ala↓Asp, mediated by BMP-1. The expression of this protease as well as the expression of two key enzymes of the carotinoid metabolism, the β,β-carotene-15,15'-dioxygenase and the retinal dehydrogenase/reductase, were found to be strongly up-regulated by retinoic acid. Hence retinoic acid turned out to be a key factor in skeletogenesis in the most ancient still existing metazoans, the sponges. GENERAL SIGNIFICANCE: It is shown that retinoic acid regulates the formation of the organic cylinder that surrounds the axis of the spicules and enables, as a scaffold, and allows the radial apposition of new silica layers and hence the growth of the spicules. |
| | |
Authors:
|
Werner E G Müller; Michael Binder; Johannes von Lintig; Yue-Wei Guo; Xiaohong Wang; Jaap A Kaandorp; Matthias Wiens; Heinz C Schröder |
Related Documents
:
|
15734723 - Acute central infusion of leptin modulates fatty acid mobilization by affecting lipolys... 7016633 - Prolactin and the regulation of adipose-tissue metabolism during lactation in rats. 15498973 - Compensated deleterious mutations in insect genomes. |
Publication Detail:
|
Type: - Date: 2011-9-16 |
Journal Detail:
|
Title: Biochimica et biophysica acta Volume: - ISSN: 0006-3002 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
|
Created Date: 2011-9-28 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011. Published by Elsevier B.V. |
Affiliation:
|
ERC Advanced Grant Research Group, Institute for Physiological Chemistry, University Medical Center of the Johannes Gutenberg University Mainz, Duesbergweg 6, D-55128 Mainz, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Ancient diversity of splicing motifs and protein surfaces in the wild emmer wheat (Triticum dicoccoi...
Next Document: Graphdiyne: a versatile nanomaterial for electronics and hydrogen purification.