Document Detail

Interaction of porcine vasoactive intestinal peptide with dispersed pancreatic acinar cells from the guinea pig. Structural requirements for effects of vasoactive intestinal peptide and secretin on cellular adenosine 3':5'-monophosphate.
MedLine Citation:
PMID:  181379     Owner:  NLM     Status:  MEDLINE    
Secretin and vasoactive intestinal peptide (VIP), but not glucagon, stimulate accumulation of cyclic AMP in dispersed guinea pig pancreatic acinar cells. Secretin stimulated cellular accumulation of cyclic AMP by interacting with a single class of high affinity receptors. On the other hand, the dose-response curve for VIP-stimulated cellular cyclic AMP was biphasic and reflected interaction of this peptide with two classes of receptors. Results obtained with synthetic fragments of VIP and secretin indicate that the receptor having a high affinity for VIP has a low affinity for secretin, interacts with, but does not distinguish among, secretin, secretin 5-27 and [6-tyrosine] secretin or among secretin 14-27, VIP 14-28, VIP 15-28, and increases cellular cyclic AMP when occupied by VIP, but not when occupied by secretin, [6-tyrosine] secretin, or secretin 1-14. The receptor having a low affinity for VIP has a high affinity for secretin, interacts with and distinguishes among secretin, secretin 5-27, and [6-tyrosine] secretin, interacts with secretin 14-27 but not with VIP 14-28 or VIP 15-28, and increases cellular cyclic AMP when occupied by VIP, secretin, [6-tyrosine] secretin, or secretin 1-14.
P Robberecht; T P Conlon; J D Gardner
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  251     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1976 Aug 
Date Detail:
Created Date:  1976-10-01     Completed Date:  1976-10-01     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4635-9     Citation Subset:  IM    
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MeSH Terms
Binding Sites
Cyclic AMP / metabolism*
Gastrointestinal Hormones* / pharmacology
Guinea Pigs
Pancreas / cytology,  drug effects,  metabolism*
Peptide Fragments / pharmacology
Protein Binding
Receptors, Drug
Secretin* / pharmacology
Reg. No./Substance:
0/Gastrointestinal Hormones; 0/Peptide Fragments; 0/Receptors, Drug; 1393-25-5/Secretin; 60-92-4/Cyclic AMP

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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