Document Detail


Interaction of porcine vasoactive intestinal peptide with dispersed pancreatic acinar cells from the guinea pig. Structural requirements for effects of vasoactive intestinal peptide and secretin on cellular adenosine 3':5'-monophosphate.
MedLine Citation:
PMID:  181379     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Secretin and vasoactive intestinal peptide (VIP), but not glucagon, stimulate accumulation of cyclic AMP in dispersed guinea pig pancreatic acinar cells. Secretin stimulated cellular accumulation of cyclic AMP by interacting with a single class of high affinity receptors. On the other hand, the dose-response curve for VIP-stimulated cellular cyclic AMP was biphasic and reflected interaction of this peptide with two classes of receptors. Results obtained with synthetic fragments of VIP and secretin indicate that the receptor having a high affinity for VIP has a low affinity for secretin, interacts with, but does not distinguish among, secretin, secretin 5-27 and [6-tyrosine] secretin or among secretin 14-27, VIP 14-28, VIP 15-28, and increases cellular cyclic AMP when occupied by VIP, but not when occupied by secretin, [6-tyrosine] secretin, or secretin 1-14. The receptor having a low affinity for VIP has a high affinity for secretin, interacts with and distinguishes among secretin, secretin 5-27, and [6-tyrosine] secretin, interacts with secretin 14-27 but not with VIP 14-28 or VIP 15-28, and increases cellular cyclic AMP when occupied by VIP, secretin, [6-tyrosine] secretin, or secretin 1-14.
Authors:
P Robberecht; T P Conlon; J D Gardner
Related Documents :
6379759 - Vasoactive intestinal polypeptide (vip) in the pig pancreas: role of vipergic nerves in...
7045549 - Evaluation of aldosterone antagonists in healthy man.
23389339 - Metabolic fluxes in schizosaccharomyces pombe grown on glucose and mixtures of glycerol...
23795479 - Biochemical parameters of saccharopolyspora erythraea during feeding ammonium sulphate ...
23874849 - Fermentation of xylose causes inefficient metabolic state due to carbon/energy starvati...
24062879 - Ethanol and acetate acting as carbon/energy sources negatively affect yeast chronologic...
9099819 - Immediate and delayed effects of in vitro ischemia on glutamate efflux from guinea-pig ...
17432269 - Methods, applications and concepts of metabolite profiling: secondary metabolism.
23840289 - Correction: relative metabolic stability, but disrupted circadian cortisol secretion du...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  251     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1976 Aug 
Date Detail:
Created Date:  1976-10-01     Completed Date:  1976-10-01     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4635-9     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Binding Sites
Cyclic AMP / metabolism*
Gastrointestinal Hormones* / pharmacology
Guinea Pigs
Kinetics
Male
Pancreas / cytology,  drug effects,  metabolism*
Peptide Fragments / pharmacology
Protein Binding
Receptors, Drug
Secretin* / pharmacology
Swine
Chemical
Reg. No./Substance:
0/Gastrointestinal Hormones; 0/Peptide Fragments; 0/Receptors, Drug; 1393-25-5/Secretin; 60-92-4/Cyclic AMP

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Detection of an essential sulfhydryl group in phosphoglycerate mutase with an affinity-labeling reag...
Next Document:  Action of cholecystokinin, cholinergic agents, and A-23187 on accumulation of guanosine 3':5'-monoph...