Document Detail


Interaction of influenza and parainfluenza viruses with polycations, organic oligocations and chromosome preparations.
MedLine Citation:
PMID:  6151349     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
As evaluated by light scattering at 90 degrees, natural organic oligocations such as putrescine, spermidine and spermine interfered with myxovirus aggregates which were induced by the histone H2A and strongly amplified by shaking during incubation. In contrast, the synthetic oligocation 1.7-diamino heptane itself aggregated the virus particles, its action being unmodified by adding polycationic H2A in abundance. When human chromosome preparations treated with protamine solution and shaked during incubation were covered with a stable polycationic molecular layer, the chromosomes had become unstainable by the Giemsa method even if the dye was used in excess. Nevertheless, the affinity of influenza virus particles for protamine was so high that they were able to dissociate the protamine molecules from the preformed complexes reconstituting the affinity of chromosome preparations to Giemsa stain. The virus-caused shift in the staining ability of chromosomes did not occur when bacterial suspension was added instead of the viral one. The model of oligocationic relaxation and of polycation condensation accounting for the modulatory effects of polyamines is discussed.
Authors:
S Antohi; D Rojanschi; O Hörer; V Brumfeld; A Petrescu; N Cajal
Related Documents :
12163909 - Evidence of spotted fever group rickettsiae in state of rio de janeiro, brazil.
3377279 - Preliminary evidence for a fourth allele at the phosphohexose isomerase (phi) locus of ...
6200639 - Amplified kpnl repetitive dna sequences in homogeneously staining regions of a human me...
24564389 - Sex, genetics, and the control of thyroxine and thyrotropin in mice.
10997779 - Combined pi-dapi staining (cpd) reveals nor asymmetry and facilitates karyotyping of pl...
2456279 - Harmine as a substitute for 33258 hoechst in the fpg technique.
8661129 - A new region of conservation is defined between human and mouse x chromosomes.
10211989 - Significance of trisomy 7 and 12 in thyroid lesions with follicular differentiation: a ...
9604759 - Chromosomal mosaicism in cleavage-stage human embryos and the accuracy of single-cell g...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta virologica     Volume:  28     ISSN:  0001-723X     ISO Abbreviation:  Acta Virol.     Publication Date:  1984 Sep 
Date Detail:
Created Date:  1985-01-25     Completed Date:  1985-01-25     Revised Date:  2001-11-02    
Medline Journal Info:
Nlm Unique ID:  0370401     Medline TA:  Acta Virol     Country:  CZECHOSLOVAKIA    
Other Details:
Languages:  eng     Pagination:  362-8     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cations / pharmacology*
Chromosomes / physiology
Histones / pharmacology
Influenza A virus / drug effects*
Light
Parainfluenza Virus 1, Human / drug effects*
Polyamines / pharmacology
Polymers / pharmacology
Scattering, Radiation
Chemical
Reg. No./Substance:
0/Cations; 0/Histones; 0/Polyamines; 0/Polymers; 0/polycations

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Cleavage of mouse ribosomal RNAs by the endonuclease associated with Newcastle disease virus.
Next Document:  Ts mutations in the genome of cold-adapted variants of human influenza virus A/Krasnodar/101/59 (H2N...