Document Detail


Interaction of glucuronic acid and iduronic acid-rich glycosaminoglycans and their modified forms with hydroxyapatite.
MedLine Citation:
PMID:  11761169     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Proteoglycans and their spatial arms, glycosaminoglycans (GAGs), are known to interact with hydroxyapatite (HAP) and have been implicated as important modulators of mineralisation. In the present study isotherm data (0.02 M sodium acetate, pH 6.8) revealed that the iduronic-rich GAGs heparan sulphate, heparin and dermatan sulphate showed greater binding onto HAP with higher adsorption maxima compared with the glucuronic acid-rich GAGs chondroitin-4-sulphate, chondroitin-6-sulphate and hyaluronan. Chemically desulphated chondroitin showed no adsorption onto HAP. With the exception of hyaluronan, the GAGs studied showed no desorbability in sodium acetate buffer only, whereas in di-sodium orthophosphate, desorption occurred much more readily. The data indicates that GAG chemistry and conformation in solution greatly influence the interaction of these molecules with HAP. The conformational flexibility of iduronic acid residues may be an important determinant in the strong binding of iduronic acid-rich GAGs to HAP, increasing the possibility of the appended anionic groups matching calcium sites on the HAP surface, compared with more rigid glucuronic acid residues. This work provides important information concerning interfacial adsorption phenomena between the organic-inorganic phases of mineralised systems.
Authors:
Sarah G Rees; WassellDianaT Hughes; Graham Embery
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomaterials     Volume:  23     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2001-12-11     Completed Date:  2002-05-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  481-9     Citation Subset:  IM    
Affiliation:
Department of Basic Dental Science, Dental School, University of Wales College of Medicine, Cardiff, UK. reessg1@cardiff.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Adsorption
Glucuronic Acid / chemistry*
Glycosaminoglycans / chemistry*
Hydroxyapatites / chemistry*
Iduronic Acid / chemistry*
Thermodynamics
Chemical
Reg. No./Substance:
0/Glycosaminoglycans; 0/Hydroxyapatites; 3402-98-0/Iduronic Acid; 576-37-4/Glucuronic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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