Document Detail

Interaction of fish aryl hydrocarbon receptor paralogs (AHR1 and AHR2) with the retinoblastoma protein.
MedLine Citation:
PMID:  19539383     Owner:  NLM     Status:  MEDLINE    
The aryl hydrocarbon receptor (AHR) mediates the toxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and related compounds. In some mammalian cell lines, TCDD induces G1 cell cycle arrest, which depends on an interaction between the AHR and the retinoblastoma tumor suppressor (RB). Mammals possess one AHR, whereas fishes possess two or more AHR paralogs that differ in the domains important for AHR-RB interactions in mammals. To test the hypothesis that fish AHR paralogs differ in their ability to interact with RB, we cloned RB cDNA from Atlantic killifish, Fundulus heteroclitus, and studied the interactions of killifish RB protein with killifish AHR1 and AHR2. In coimmunoprecipitation experiments, in vitro-expressed killifish RB coprecipitated with both AHR1 and AHR2. Consistent with these results, both killifish AHR1 and AHR2 interacted with RB in mammalian two-hybrid assays. These results suggest that both fish AHR1 and AHR2 paralogs may have the potential to influence cell proliferation through interactions with RB.
Rebeka R Merson; Sibel I Karchner; Mark E Hahn
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-06-02
Journal Detail:
Title:  Aquatic toxicology (Amsterdam, Netherlands)     Volume:  94     ISSN:  1879-1514     ISO Abbreviation:  Aquat. Toxicol.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-24     Completed Date:  2009-08-17     Revised Date:  2014-09-08    
Medline Journal Info:
Nlm Unique ID:  8500246     Medline TA:  Aquat Toxicol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  47-55     Citation Subset:  IM    
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MeSH Terms
Amino Acid Sequence
Base Sequence
COS Cells
Cercopithecus aethiops
Cloning, Molecular
Fundulidae / genetics*,  metabolism*
Gene Expression Regulation
Molecular Sequence Data
Receptors, Aryl Hydrocarbon / metabolism*
Retinoblastoma Protein / chemistry,  genetics,  metabolism*
Sequence Alignment
Grant Support
F32 ES005935/ES/NIEHS NIH HHS; F32 ES005935/ES/NIEHS NIH HHS; F32 ES005935-01/ES/NIEHS NIH HHS; K22 ES014736/ES/NIEHS NIH HHS; K22 ES014736/ES/NIEHS NIH HHS; K22 ES014736-01/ES/NIEHS NIH HHS; P20 RR016457/RR/NCRR NIH HHS; P20 RR016457-076995/RR/NCRR NIH HHS; P20RR016457/RR/NCRR NIH HHS; P42 ES007381-140021/ES/NIEHS NIH HHS; P42ES007381/ES/NIEHS NIH HHS
Reg. No./Substance:
0/Receptors, Aryl Hydrocarbon; 0/Retinoblastoma Protein

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