Document Detail

Interaction of dolichol and dolichyl phosphate with phospholipid bilayers.
MedLine Citation:
PMID:  6547619     Owner:  NLM     Status:  MEDLINE    
The thermotropic phase transition of dipalmitoylphosphatidylcholine vesicles reconstituted with dolichol or dolichyl phosphate was investigated as a function of the lipid-to-polyisoprenoid ratio by means of differential scanning calorimetry and fluorescence depolarization of the embedded probe 1,6-diphenyl-1,3,5-hexatriene. At the concentrations studied, dolichol and dolichyl phosphate lowered and broadened the transition temperature of dipalmitoylphosphatidylcholine bilayers. Dolichol was found to increase the motional freedom of the bilayer both below and above the transition temperature as determined by fluorescence depolarization. In contrast, low concentrations of dolichyl phosphate decreased the bilayer motional freedom below the transition temperature while high concentrations increased the motional freedom. Above the transition temperature, dolichyl phosphate decreased bilayer 'fluidity' at all concentrations. The data suggest that these polyisoprenoids perturb the bilayer lattice, with the neutral species dolichol increasing membrane 'fluidity', while dolichyl phosphate acts to 'stiffen' the membrane.
C Vigo; S H Grossman; W Drost-Hansen
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  774     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1984 Jul 
Date Detail:
Created Date:  1984-09-06     Completed Date:  1984-09-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  221-6     Citation Subset:  IM    
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MeSH Terms
Calorimetry, Differential Scanning
Diterpenes / metabolism*
Dolichol / metabolism*
Dolichol Phosphates / metabolism*
Fluorescence Polarization
Lipid Bilayers / metabolism*
Membrane Fluidity
Polyisoprenyl Phosphates / metabolism*
Pulmonary Surfactants / metabolism
Grant Support
Reg. No./Substance:
0/Diterpenes; 0/Dolichol Phosphates; 0/Lipid Bilayers; 0/Polyisoprenyl Phosphates; 0/Pulmonary Surfactants; 12698-55-4/dolichol monophosphate; 1720-32-7/Diphenylhexatriene; 2067-66-5/Dolichol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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