| Interaction of dolichol and dolichyl phosphate with phospholipid bilayers. | |
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MedLine Citation:
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PMID: 6547619 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The thermotropic phase transition of dipalmitoylphosphatidylcholine vesicles reconstituted with dolichol or dolichyl phosphate was investigated as a function of the lipid-to-polyisoprenoid ratio by means of differential scanning calorimetry and fluorescence depolarization of the embedded probe 1,6-diphenyl-1,3,5-hexatriene. At the concentrations studied, dolichol and dolichyl phosphate lowered and broadened the transition temperature of dipalmitoylphosphatidylcholine bilayers. Dolichol was found to increase the motional freedom of the bilayer both below and above the transition temperature as determined by fluorescence depolarization. In contrast, low concentrations of dolichyl phosphate decreased the bilayer motional freedom below the transition temperature while high concentrations increased the motional freedom. Above the transition temperature, dolichyl phosphate decreased bilayer 'fluidity' at all concentrations. The data suggest that these polyisoprenoids perturb the bilayer lattice, with the neutral species dolichol increasing membrane 'fluidity', while dolichyl phosphate acts to 'stiffen' the membrane. |
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Authors:
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C Vigo; S H Grossman; W Drost-Hansen |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 774 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1984 Jul |
Date Detail:
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Created Date: 1984-09-06 Completed Date: 1984-09-06 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 221-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Calorimetry, Differential Scanning Diphenylhexatriene Diterpenes / metabolism* Dolichol / metabolism* Dolichol Phosphates / metabolism* Fluorescence Polarization Lipid Bilayers / metabolism* Mathematics Membrane Fluidity Polyisoprenyl Phosphates / metabolism* Pulmonary Surfactants / metabolism Temperature |
| Grant Support | |
ID/Acronym/Agency:
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CA 28781/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Diterpenes; 0/Dolichol Phosphates; 0/Lipid Bilayers; 0/Polyisoprenyl Phosphates; 0/Pulmonary Surfactants; 12698-55-4/dolichol monophosphate; 1720-32-7/Diphenylhexatriene; 2067-66-5/Dolichol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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