| Interaction of contractile activity and training history on mRNA abundance in skeletal muscle from trained athletes. | |
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MedLine Citation:
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PMID: 16338907 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Skeletal muscle displays enormous plasticity to respond to contractile activity with muscle from strength- (ST) and endurance-trained (ET) athletes representing diverse states of the adaptation continuum. Training adaptation can be viewed as the accumulation of specific proteins. Hence, the altered gene expression that allows for changes in protein concentration is of major importance for any training adaptation. Accordingly, the aim of the present study was to quantify acute subcellular responses in muscle to habitual and unfamiliar exercise. After 24-h diet/exercise control, 13 male subjects (7 ST and 6 ET) performed a random order of either resistance (8 x 5 maximal leg extensions) or endurance exercise (1 h of cycling at 70% peak O2 uptake). Muscle biopsies were taken from vastus lateralis at rest and 3 h after exercise. Gene expression was analyzed using real-time PCR with changes normalized relative to preexercise values. After cycling exercise, peroxisome proliferator-activated receptor-gamma coactivator-1alpha (ET approximately 8.5-fold, ST approximately 10-fold, P < 0.001), pyruvate dehydrogenase kinase-4 (PDK-4; ET approximately 26-fold, ST approximately 39-fold), vascular endothelial growth factor (VEGF; ET approximately 4.5-fold, ST approximately 4-fold), and muscle atrophy F-box protein (MAFbx) (ET approximately 2-fold, ST approximately 0.4-fold) mRNA increased in both groups, whereas MyoD (approximately 3-fold), myogenin (approximately 0.9-fold), and myostatin (approximately 2-fold) mRNA increased in ET but not in ST (P < 0.05). After resistance exercise PDK-4 (approximately 7-fold, P < 0.01) and MyoD (approximately 0.7-fold) increased, whereas MAFbx (approximately 0.7-fold) and myostatin (approximately 0.6-fold) decreased in ET but not in ST. We conclude that prior training history can modify the acute gene responses in skeletal muscle to subsequent exercise. |
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Authors:
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Vernon G Coffey; Anthony Shield; Benedict J Canny; Kate A Carey; David Cameron-Smith; John A Hawley |
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Publication Detail:
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Type: Journal Article; Randomized Controlled Trial Date: 2005-12-06 |
Journal Detail:
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Title: American journal of physiology. Endocrinology and metabolism Volume: 290 ISSN: 0193-1849 ISO Abbreviation: Am. J. Physiol. Endocrinol. Metab. Publication Date: 2006 May |
Date Detail:
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Created Date: 2006-04-10 Completed Date: 2006-06-19 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 100901226 Medline TA: Am J Physiol Endocrinol Metab Country: United States |
Other Details:
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Languages: eng Pagination: E849-55 Citation Subset: IM |
Affiliation:
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School of Medical Sciences, RMIT University, Melbourne, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Bicycling / physiology Cross-Over Studies Gene Expression / genetics Heat-Shock Proteins / genetics Humans Male Muscle Contraction / physiology* Muscle Proteins / genetics Muscle, Skeletal / physiology* MyoD Protein / genetics Myogenin / genetics Myostatin Protein Kinases / genetics RNA, Messenger / genetics, metabolism* SKP Cullin F-Box Protein Ligases / genetics Sports / physiology* Transcription Factors / genetics Transforming Growth Factor beta / genetics Vascular Endothelial Growth Factor A / genetics Weight Lifting / physiology |
| Chemical | |
Reg. No./Substance:
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0/Heat-Shock Proteins; 0/MSTN protein, human; 0/Muscle Proteins; 0/MyoD Protein; 0/Myogenin; 0/Myostatin; 0/PPARGC1A protein, human; 0/RNA, Messenger; 0/Transcription Factors; 0/Transforming Growth Factor beta; 0/Vascular Endothelial Growth Factor A; EC 2.7.-/Protein Kinases; EC 2.7.1.-/pyruvate dehydrogenase kinase 4; EC 6.3.2.19/FBXO32 protein, human; EC 6.3.2.19/SKP Cullin F-Box Protein Ligases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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