Document Detail


Interaction of cationic ultrasmall superparamagnetic iron oxide nanoparticles with human melanoma cells.
MedLine Citation:
PMID:  20874022     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ultrasmall superparamagnetic iron oxide nanoparticles (USPIONs) are currently under development for the intracellular delivery of therapeutics. However, the mechanisms of cellular uptake and the cellular reaction to this uptake, independent of therapeutics, are not well defined. The interactions of biocompatible cationic aminoUSPIONs with human cells was studied in 2D and 3D cultures using biochemical and electron microscopy techniques. AminoUSPIONs were internalized by human melanoma cells in 2D and 3D cultures. Uptake was clathrin mediated and the particles localized in lysosomes, inducing activation of the lysosomal cathepsin D and decreasing the expression of the transferrin receptor in human melanoma cells and/or skin fibroblasts. AminoUSPIONs deeply invaded 3D spheroids of human melanoma cells. Thus, aminoUSPIONs can invade tumors and their uptake by human cells induces cell reaction.
Authors:
Feride Cengelli; Francine Voinesco; Lucienne Juillerat-Jeanneret
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nanomedicine (London, England)     Volume:  5     ISSN:  1748-6963     ISO Abbreviation:  Nanomedicine (Lond)     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-29     Completed Date:  2011-01-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101278111     Medline TA:  Nanomedicine (Lond)     Country:  England    
Other Details:
Languages:  eng     Pagination:  1075-87     Citation Subset:  IM    
Affiliation:
Centre Hospitalier Universitaire Vaudois & University of Lausanne, Lausanne, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Cations
Cell Line, Tumor
DNA Primers
Ferric Compounds / metabolism*
Humans
Melanoma / pathology*
Metal Nanoparticles*
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/Cations; 0/DNA Primers; 0/Ferric Compounds; 1309-37-1/ferric oxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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