Document Detail


Interaction of bovine serum albumin with some novel PEG-containing diblock copolymers.
MedLine Citation:
PMID:  18602419     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A comparative study on the interaction of different PEG-containing diblock copolymers including SA400, SA600, SA1500 and OA1500 (stearyl and oleyl esters of polyethylene glycol with 400, 600 and 1500 molecular weights, respectively) with bovine serum albumin (BSA) was carried out using isothermal titration calorimetry (ITC), attenuated total reflectance Fourier transform infrared (ATR-FTIR), circular dichroism (CD), and fluorescence spectroscopies. ITC data show that SA400, SA600, SA1500 and OA1500 bind to BSA, with association constants of (14.5, 3.16, 50.7 and 17.6)x10(3) M(-1), respectively. Results also show that the binding is enthalpically driven, disfavored by conformational entropy. Quantitative analysis of the FTIR absorbance spectra at amide I' (1600-1700 cm(-1)) as well as far UV circular dichroism data show that these polymers do not disturb the BSA structure and only cause a slight increment in helicity along with a slight decrease in the beta-structure. Only stearyl esters SA400 and SA1500 slightly decreased the random structure content of the BSA. The diblock copolymers inhibit protein aggregation and bind to BSA better than their constituent PEGs causing an increment in its Tm; SA1500 is showing the strongest effect.
Authors:
Asadollah Asadi; Ali A Saboury; A A Moosavi-Movahedi; A Divsalar; Mohammad N Sarbolouki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-17
Journal Detail:
Title:  International journal of biological macromolecules     Volume:  43     ISSN:  0141-8130     ISO Abbreviation:  Int. J. Biol. Macromol.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-08-26     Completed Date:  2008-11-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7909578     Medline TA:  Int J Biol Macromol     Country:  England    
Other Details:
Languages:  eng     Pagination:  262-70     Citation Subset:  IM    
Affiliation:
Biophysics Department, Institute of Biochemistry and Biophysics, A University of Tehran, P.O. Box 13145-1384, Tehran, Iran.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calorimetry
Cattle
Circular Dichroism
Hydrophobicity
Micelles
Molecular Weight
Oleic Acid / chemistry
Polyethylene Glycols / chemistry*,  pharmacology
Protein Binding
Protein Conformation / drug effects
Protein Denaturation / drug effects
Serum Albumin, Bovine / chemistry*
Spectrometry, Fluorescence
Spectroscopy, Fourier Transform Infrared
Stearic Acids / chemistry
Temperature
Thermodynamics
Titrimetry
Chemical
Reg. No./Substance:
0/Micelles; 0/Polyethylene Glycols; 0/Serum Albumin, Bovine; 0/Stearic Acids; 112-80-1/Oleic Acid; 57-11-4/stearic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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