| Interaction between ubiquitin-protein ligase SCFSKP2 and E2F-1 underlies the regulation of E2F-1 degradation. | |
| | |
MedLine Citation:
|
PMID: 10559858 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The transcription factor E2F-1 is important in the control of cell proliferation. Its activity must be tightly regulated in a cell-cycle-dependent manner to enable programs of gene expression to be coupled closely with cell-cycle position. Here we show that, following its accumulation in the late G1 phase of the cell cycle, E2F-1 is rapidly degraded in S/G2 phase. This event is linked to a specific interaction of E2F-1 with the F-box-containing protein p45SKP2, which is the cell-cycle-regulated component of the ubiquitin-protein ligase SCFSKP2 that recognizes substrates for this ligase. Disruption of the interaction between E2F-1 and p45SKP2 results in a reduction in ubiquitination of E2F-1 and the stabilization and accumulation of transcriptionally active E2F-1 protein. These results indicate that an SCFSKP2-dependent ubiquitination pathway may be involved in the downregulation of E2F-1 activity in the S/G2 phase of the cell cycle, and suggest a link between SCFSKP2 and cell-cycle-dependent gene control. |
| | |
Authors:
|
A Marti; C Wirbelauer; M Scheffner; W Krek |
Related Documents
:
|
19638578 - Prolonged cell cycle response of hela cells to low-level alkylation exposure. 18267078 - Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. 6341358 - Cell cycle parameters of slowly growing escherichia coli b/r studied by flow cytometry. 7846128 - Life, death and genomic change in perturbed cell cycles. 10554018 - Apoptotic conversion: evidence for exchange of genetic information between prostate can... 21206908 - Label-free determination of the number of biomolecules attached to cells by measurement... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Nature cell biology Volume: 1 ISSN: 1465-7392 ISO Abbreviation: Nat. Cell Biol. Publication Date: 1999 May |
Date Detail:
|
Created Date: 1999-12-07 Completed Date: 1999-12-07 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 100890575 Medline TA: Nat Cell Biol Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 14-9 Citation Subset: IM |
Affiliation:
|
Friedrich Miescher Institut, Basel, Switzerland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
3T3 Cells Animals Carrier Proteins* Cell Cycle / physiology* Cell Cycle Proteins / metabolism* Cell Line DNA-Binding Proteins* E2F Transcription Factors E2F1 Transcription Factor G1 Phase G2 Phase Hela Cells Homeostasis Humans Kinetics Ligases / metabolism* Mice Retinoblastoma-Binding Protein 1 S Phase S-Phase Kinase-Associated Proteins Substrate Specificity Transcription Factor DP1 Transcription Factors / metabolism* Ubiquitin-Protein Ligases Ubiquitins / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Arid4a protein, mouse; 0/Carrier Proteins; 0/Cell Cycle Proteins; 0/DNA-Binding Proteins; 0/E2F Transcription Factors; 0/E2F1 Transcription Factor; 0/E2F1 protein, human; 0/E2f1 protein, mouse; 0/Retinoblastoma-Binding Protein 1; 0/S-Phase Kinase-Associated Proteins; 0/Transcription Factor DP1; 0/Transcription Factors; 0/Ubiquitins; EC 6.-/Ligases; EC 6.3.2.19/Ubiquitin-Protein Ligases |
| Comments/Corrections | |
Comment In:
|
Nat Cell Biol. 1999 May;1(1):E5-7
[PMID:
10559870
]
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Cdc42 controls secretory and endocytic transport to the basolateral plasma membrane of MDCK cells.
Next Document: Nucleolar Arf sequesters Mdm2 and activates p53.