Document Detail

Interaction between two different regulatory elements activates the murine alpha A-crystallin gene promoter in explanted lens epithelia.
MedLine Citation:
PMID:  3474517     Owner:  NLM     Status:  MEDLINE    
Previous experiments have indicated that 5' flanking DNA sequences (nucleotides-366 to +46) are capable of regulating the lens-specific transcription of the murine alpha A-crystallin gene. Here we have analyzed these 5' regulatory sequences by transfecting explanted embryonic chicken lens epithelia with different alpha A-crystallin-CAT (chloramphenicol acetyltransferase) hybrid genes (alpha A-crystallin promoter sequences fused to the bacterial CAT gene in the pSVO-CAT expression vector). The results indicated the presence of a proximal (-88 to +46) and a distal (-111 to -88) domain which must interact for promoter function. Deletion experiments showed that the sequence between -88 and -60 was essential for function of the proximal domain in the explanted epithelia. A synthetic oligonucleotide containing the sequence between -111 and -84 activated the proximal domain when placed in either orientation 57 base pairs upstream from position -88 of the alpha A-crystallin-CAT hybrid gene.
A B Chepelinsky; B Sommer; J Piatigorsky
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  7     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  1987 May 
Date Detail:
Created Date:  1987-07-31     Completed Date:  1987-07-31     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1807-14     Citation Subset:  IM    
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MeSH Terms
Acetyltransferases / genetics
Chloramphenicol O-Acetyltransferase
Chromosome Mapping
Crystallins / genetics*
Epithelium / physiology
Gene Expression Regulation
Genes, Regulator*
Lens, Crystalline / physiology*
Promoter Regions, Genetic*
Reg. No./Substance:
0/Crystallins; EC 2.3.1.-/Acetyltransferases; EC O-Acetyltransferase

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