Document Detail


Interaction between thromboxane and free radical mechanisms in experimental ischaemic rabbit skin flaps.
MedLine Citation:
PMID:  2089538     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The possible relationship between increased blood levels of thromboxane (TXA2) and tissue levels of free radicals during ischaemia was investigated. Rabbit epigastric skin flaps were subjected to 4 h of body temperature ischaemia, then infused with either the TXA2 synthetase inhibitor UK-38,485, the free radical scavenger superoxide dismutase (SOD), or both immediately prior to reperfusion. After 30 min of reperfusion, increases in the tissue levels of xanthine oxidase (XO) and malonyldialdehyde (MDA), both of which are indices of free radical generation and decreases in the tissue levels of SOD were found. SOD treatment completely restored XO, MDA and SOD levels to normal, whereas UK-38,485 only partially improved all three parameters. None of these changes was statistically significant. Effluent blood thromboxane B2 (TXB2) levels from the flap increased significantly (P less than 0.01) after ischaemia and were reduced significantly by both UK-38,485 and SOD (P less than 0.05). Combined UK-38,485 and SOD treatment was no better than treatment with either agent alone. ATP levels and oedema, which decreased and increased respectively due to ischaemia, were not significantly altered by drug infusion. These results suggest that free radical damage may be related to TXA2-generated thrombosis in ischaemia/reperfusion injury.
Authors:
K R Knight; C G Mellow; P A Abbey; M F Angel; D A Lepore; L I Arnold; K A Gray; B M O'Brien
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Research in experimental medicine. Zeitschrift für die gesamte experimentelle Medizin einschliesslich experimenteller Chirurgie     Volume:  190     ISSN:  0300-9130     ISO Abbreviation:  Res Exp Med (Berl)     Publication Date:  1990  
Date Detail:
Created Date:  1991-05-28     Completed Date:  1991-05-28     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0324736     Medline TA:  Res Exp Med (Berl)     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  423-33     Citation Subset:  IM    
Affiliation:
Microsurgery Research Centre, St. Vincent's Hospital, Fitzroy, Victoria, Australia.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism
Animals
Body Water / metabolism
Free Radicals
Imidazoles / pharmacology
Ischemia / metabolism*
Malondialdehyde / metabolism
Rabbits
Reperfusion Injury / drug therapy,  metabolism
Skin / blood supply*,  metabolism
Superoxide Dismutase / pharmacology
Surgical Flaps
Thromboxane-A Synthase / antagonists & inhibitors
Thromboxanes / metabolism*
Xanthine Oxidase / metabolism
Chemical
Reg. No./Substance:
0/Free Radicals; 0/Imidazoles; 0/Thromboxanes; 542-78-9/Malondialdehyde; 56-65-5/Adenosine Triphosphate; 76894-77-4/dazmegrel; EC 1.15.1.1/Superoxide Dismutase; EC 1.17.3.2/Xanthine Oxidase; EC 5.3.99.5/Thromboxane-A Synthase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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