Document Detail


Interaction between season and culture with insulin-like growth factor-1 on survival of in vitro produced embryos following transfer to lactating dairy cows.
MedLine Citation:
PMID:  17452048     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Culture of bovine embryos in the presence of insulin-like growth factor-1 (IGF-1) can increase pregnancy rates following transfer to heat-stressed, lactating dairy cows. The objective of the present experiment was to determine whether the effect of IGF-1 on post-transfer embryo survival was a general effect or one specific to heat stress. Lactating recipients (n=311) were synchronized for timed-embryo transfer at four locations. Embryos were produced in vitro and cultured with or without 100 ng/mL IGF-1. At Day 7 after anticipated ovulation (Day 0), a single embryo was randomly transferred to each recipient. Pregnancy was diagnosed at Day 21 by elevated plasma progesterone concentrations, at Days 27-32 by ultrasonography, and at Days 41-49 by transrectal palpation. Transfers were categorized into two seasons, hot or cool (based on the month of transfer). There was a tendency (P<0.09) for an interaction between embryo treatment and season for pregnancy rate at Day 21; this interaction was significant at Days 30 and 45 (P<0.02). Recipients receiving IGF-1 treated embryos had higher pregnancy rates in the hot season but not in the cool season. There was a similar interaction between embryo treatment and season for overall calving rate (P<0.05). There was also an interaction between season and treatment affecting pregnancy loss between Days 21 and 30; recipients that received IGF-1 treated embryos had less pregnancy loss during this time period in the hot season but not in the cool season. The overall proportion of male calves born was 77.5%. In conclusion, treatment of embryos with IGF-1 improved pregnancy and calving rates following the transfer of in vitro produced embryos into lactating recipients, but only under heat-stress conditions.
Authors:
J Block; P J Hansen
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-04-23
Journal Detail:
Title:  Theriogenology     Volume:  67     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-07     Completed Date:  2007-10-31     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1518-29     Citation Subset:  IM    
Affiliation:
Department of Animal Sciences, University of Florida, Gainesville, FL 32611-0910, USA.
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MeSH Terms
Descriptor/Qualifier:
Abortion, Veterinary / veterinary
Animals
Birth Weight / drug effects
Cattle / embryology,  physiology*
Culture Media
Embryo Culture Techniques / methods,  veterinary*
Embryo Transfer / veterinary
Embryo, Mammalian / drug effects*
Female
Fertilization in Vitro / veterinary
Insulin-Like Growth Factor I / pharmacology*
Lactation
Live Birth
Male
Pregnancy
Pregnancy Rate
Seasons*
Sex Ratio
Temperature
Time Factors
Chemical
Reg. No./Substance:
0/Culture Media; 67763-96-6/Insulin-Like Growth Factor I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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