| Interaction between the mouse homologue of CD99 and its ligand PILR as a mechanism of T cell receptor-independent thymocyte apoptosis. | |
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MedLine Citation:
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PMID: 20208422 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Here, we show that the interaction between two membrane proteins, the mouse homologue of CD99 (designated D4) and its ligand, paired immunoglobulin-like type 2 receptor (PILR), is one of the major mechanisms of thymocyte apoptosis. Using the polymeric fusion protein of PILR and IgG1 (PILR-Ig), we demonstrated that D4 ligation in the absence of T cell receptor (TCR) engagement leads to the induction of apoptosis, mainly at the double-positive stage of thymocytes. This was further confirmed by a blocking study in which blocking the interaction between D4 and PILR by soluble D4 protein led to reduced apoptosis in the fetal thymic organ culture with wild type and TCRalpha(-/-) mice. Furthermore, the dissection of intracellular signaling pathway demonstrated that D4 cross-linking led to caspase activation without any change in mitochondrial membrane potential. Based on these data, we propose a mechanism for thymocyte depletion in which the interaction between D4 and PILR delivers an active signal. |
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Authors:
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Hyo Jin Park; Young Larn Ban; Dahye Byun; Seong Hoe Park; Kyeong Cheon Jung |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental & molecular medicine Volume: 42 ISSN: 1226-3613 ISO Abbreviation: Exp. Mol. Med. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-05-31 Completed Date: 2010-08-30 Revised Date: 2010-09-30 |
Medline Journal Info:
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Nlm Unique ID: 9607880 Medline TA: Exp Mol Med Country: Korea (South) |
Other Details:
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Languages: eng Pagination: 353-65 Citation Subset: IM |
Affiliation:
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Department of Pathology, Seoul National University College of Medicine, Seoul 110-799, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Genetically Modified Antigens, CD / metabolism* Apoptosis / physiology* Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Knockout Receptors, Antigen, T-Cell / immunology, metabolism Receptors, Immunologic / genetics, immunology*, metabolism T-Lymphocytes / immunology, metabolism*, pathology* |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Cd99 protein, mouse; 0/PILRalpha protein, mouse; 0/PILRbeta protein, mouse; 0/Receptors, Antigen, T-Cell; 0/Receptors, Immunologic |
| Comments/Corrections | |
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