| Interaction between heparin and human pregnancy-associated plasma protein A (PAPP-A): a simple purification procedure. | |
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MedLine Citation:
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PMID: 6193728 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human pregnancy-associated plasma protein A (PAPP-A) binds to heparin-Sepharose. This affinity chromatography preceded by molecular sieve chromatography provides a simple two-step purification procedure of PAPP-A from late pregnancy plasma. One hundred percent of the applied PAPP-A was recovered, with more than 40% being electrophoretically homogeneous after the two procedures. The remaining PAPP-A could be purified by negative affinity chromatography on anti-total human serum immobilized on agarose. |
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Authors:
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M W Davey; B Teisner; M Sinosich; J G Grudzinskas |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Analytical biochemistry Volume: 131 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1983 May |
Date Detail:
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Created Date: 1983-10-21 Completed Date: 1983-10-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 18-24 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Affinity Chromatography, Gel Female Heparin* Humans Immunoelectrophoresis Pregnancy Pregnancy Proteins / isolation & purification* Pregnancy-Associated Plasma Protein-A / isolation & purification* Sepharose |
| Chemical | |
Reg. No./Substance:
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0/Pregnancy Proteins; 9005-49-6/Heparin; 9012-36-6/Sepharose; EC 3.4.24.-/Pregnancy-Associated Plasma Protein-A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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