Document Detail


Interaction between heparin and human pregnancy-associated plasma protein A (PAPP-A): a simple purification procedure.
MedLine Citation:
PMID:  6193728     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human pregnancy-associated plasma protein A (PAPP-A) binds to heparin-Sepharose. This affinity chromatography preceded by molecular sieve chromatography provides a simple two-step purification procedure of PAPP-A from late pregnancy plasma. One hundred percent of the applied PAPP-A was recovered, with more than 40% being electrophoretically homogeneous after the two procedures. The remaining PAPP-A could be purified by negative affinity chromatography on anti-total human serum immobilized on agarose.
Authors:
M W Davey; B Teisner; M Sinosich; J G Grudzinskas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical biochemistry     Volume:  131     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1983 May 
Date Detail:
Created Date:  1983-10-21     Completed Date:  1983-10-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  18-24     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Chromatography, Affinity
Chromatography, Gel
Female
Heparin*
Humans
Immunoelectrophoresis
Pregnancy
Pregnancy Proteins / isolation & purification*
Pregnancy-Associated Plasma Protein-A / isolation & purification*
Sepharose
Chemical
Reg. No./Substance:
0/Pregnancy Proteins; 9005-49-6/Heparin; 9012-36-6/Sepharose; EC 3.4.24.-/Pregnancy-Associated Plasma Protein-A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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