Document Detail

Interaction between eicosanoids and the complement system in salmonid fish.
MedLine Citation:
PMID:  21658408     Owner:  NLM     Status:  Publisher    
Both eicosanoid generation and the complement system have long evolutionary histories predating the emergence of the vertebrates over 500myr ago. This study investigated the interplay between these two systems in an example of a bony fish, the rainbow trout (Oncorhynchus mykiss). Specifically, it examined whether purified complement fragments including C3a-1 and zymosan-activated serum, stimulate the biosynthesis of any of these eicosanoids by trout macrophages. Incubation of macrophages with zymosan pre-incubated with normal trout serum resulted in the phagocytosis of such particles and the generation of both intra- and extra-cellularly located lipoxygenase and cyclooxygenase products. Both eicosanoid generation and phagocytosis levels were significantly elevated following incubation of zymosan in trout serum in comparison with heat-inactivated (60°C for 30min) trout serum and saline alone. A combined mass spectrometry/high performance liquid chromatography approach was employed to conclusively demonstrate the presence of the cyclooxygenase product, prostaglandin E (PGE) in the culture supernatants of ionophore-challenged macrophages. Incubation of trout macrophages with zymosan-activated trout serum (i.e. no zymosan present) failed to stimulate PGE generation. Similarly, incubation of these cells for up to 60min with C3a-1 (4 or 50nM) failed to generate significant amounts of PGE or lipoxygenase products such as leukotriene B(4/5) or lipoxin A(4/5). Longer term (6 & 24h) incubation of macrophages with C3a-1 (4nM) resulted in a time dependent increase in the generation of PGE but not leukotriene B in culture supernatants. No conclusive evidence that the increase in PGE generation was caused by changes in the expression of either cyclooxygenase-1 or -2 was found.
Andrew F Rowley; Elizabeth L Morgan; Graham W Taylor; J Oriol Sunyer; Jason W Holland; Claire L Vogan; Christopher J Secombes
Related Documents :
19641508 - Puma, a potent killer with or without p53.
8806678 - Bcl-2 and crma have different effects on transformation, apoptosis and the stability of...
24890008 - Anti-inflammatory and anti-apoptotic effect of combined treatment with methylprednisolo...
23681668 - A tspo ligand is protective in a mouse model of multiple sclerosis.
14506658 - An epithelial target site in experimental graft-versus-host disease and cytokine-mediat...
21113408 - Apoptotic regulation and trna.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-6-1
Journal Detail:
Title:  Developmental and comparative immunology     Volume:  -     ISSN:  1879-0089     ISO Abbreviation:  -     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-6-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7708205     Medline TA:  Dev Comp Immunol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier Ltd.
Department of Biosciences, College of Science, Swansea University, Swansea, SA2 8PP, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Defense of zebrafish embryos against Streptococcus pneumoniae infection is dependent on the phagocyt...
Next Document:  Neuroprotection of ?-synuclein under acute and chronic rotenone and maneb treatment is abolished by ...