Document Detail


Interaction between clenbuterol and run training: effects on exercise performance and MLC isoform content.
MedLine Citation:
PMID:  8964739     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of this study was to determine the separate and combined effects of clenbuterol (CB) administration and interval training on running performance and myosin light-chain (MLC) isoform expression in mouse skeletal muscle. Mice were randomly assigned to one of four treatment groups: 1) control (Con), 2) exercise (Ex), 3) drug (CB), or 4) exercise + drug (Ex + CB). CB and Ex + CB mice were given CB (1.6 mg/kg) orally 4 days/wk. Ex and Ex + CB mice were trained 4 days/wk on a motorized treadmill (3 sets of 3 min, 36-40 m/min, 10-17% grade, 30-s recovery). After 8 wk of treatment, exercise conditioning increased total work performed 58% in the Ex group during a run-to-exhaustion treadmill test, whereas CB decreased total work by 25% in the CB group; in combination with exercise training, CB treatment eliminated the Ex-induced increase in work. Polyacrylamide gel electrophoresis indicated that run training, CB treatment, or a combination did not (P > 0.01) promote changes in fast and slow MLC isoforms in the soleus, gastrocnemius, or tibialis anterior muscles. Although not different from each other after 8 wk, CB and Ex + CB treatments produced significantly greater values than Con and Ex for the following variables: muscle mass (17-46%), total protein (22-50%), and myofibrillar protein (19-53%). It was concluded that CB decreases exercise performance and that the combination of Ex and CB have antagonistic effects on running performance; the two treatments do not interact to diminish the anabolic effects of CB on skeletal muscle and do not alter MLC isoform profiles.
Authors:
C P Ingalls; W S Barnes; S B Smith
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  80     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  1996 Mar 
Date Detail:
Created Date:  1996-12-03     Completed Date:  1996-12-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  795-801     Citation Subset:  IM; S    
Affiliation:
Department of Health, Texas A&M University, College Station 77843, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Weight / drug effects
Clenbuterol / pharmacology*
Gene Expression / drug effects*
Male
Mice
Mice, Inbred ICR
Muscle, Skeletal / metabolism*
Myosin Heavy Chains / genetics*
Physical Conditioning, Animal / physiology*
Time Factors
Chemical
Reg. No./Substance:
0/Myosin Heavy Chains; 37148-27-9/Clenbuterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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