Document Detail

Interaction between benzo[a]pyrene and mouse skin in organ culture.
MedLine Citation:
PMID:  6269255     Owner:  NLM     Status:  MEDLINE    
An in vitro method for studying the interaction between tritiated benzo[a]pyrene ([3H]BP) and intact mouse skin was investigated. [3H]BP was applied to the epidermal surface of the skin floating on medium, and radioactivity in the medium was measured periodically. After 48-h incubation, about 45% of the radioactivity was in the medium; of this amount, about 80% accumulated during the first 24 h. Incubation temperature, metabolic inhibitors, and the amount of BP applied caused pronounced effects on accumulation; slight to moderate effects were noted for the type and volume of medium and vehicle used. Results of the temperature and inhibitor experiments indicated that accumulation of radioactivity depended on cell viability. About 80% of the radioactivity in the medium was water soluble, showing that biotransformation of BP by skin cells occurred. We are using the skin organ culture method to help understand the interaction of mammalian skin with hydrocarbon mixtures. A study of these interactions may lead to metabolic, cytotoxic, and macromolecular-binding profiles that characterize a carcinogenic mixture of hydrocarbons.
L H Smith; J M Holland
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Toxicology     Volume:  21     ISSN:  0300-483X     ISO Abbreviation:  Toxicology     Publication Date:  1981  
Date Detail:
Created Date:  1981-11-18     Completed Date:  1981-11-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0361055     Medline TA:  Toxicology     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  47-57     Citation Subset:  IM    
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MeSH Terms
Azides / pharmacology
Benzopyrenes / pharmacology*
Culture Media
Mice, Inbred C3H
Organ Culture Techniques
Skin / drug effects*,  pathology
Reg. No./Substance:
0/Azides; 0/Benzopyrenes; 0/Culture Media; 50-32-8/Benzo(a)pyrene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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