Document Detail

Interaction between ascorbate and light-exposed riboflavin induces lung remodeling.
MedLine Citation:
PMID:  15254143     Owner:  NLM     Status:  MEDLINE    
Light-exposed parenteral multivitamins induce in lungs peroxide-like oxidant responses as well as the initiation of fibrosis. We hypothesized that peroxides generated in light-exposed total parenteral nutrition (TPN) affect lung remodeling. The objective was to assess the specific roles of peroxides, multivitamin preparation (MVP), and light exposure on lung remodeling during TPN. Three-day-old guinea pigs fitted with an indwelling catheter were assigned to the following intravenous regimens: TPN or MVP +/- photoprotection, H(2)O(2)+/- glutathione, MVP +/- metabisulfite, or ascorbic acid +/- riboflavin. Fed animals served as controls. After 4 days, lungs were sampled to determine alveolarization (intercepts), beta-actin mRNA (protection assay), and apoptosis (terminal deoxynucleotidyl transferase dUTP nick-end labeling). Data were analyzed by analysis of variance. The infusion of light-exposed multivitamins induced a 20% lower (p < 0.01) alveolarization index than fed controls, and 3-fold higher (p < 0.01) apoptotic events. This was prevented by photoprotecting TPN. The effect of multivitamins on the alveolarization index was reproduced (p < 0.05) by infusion of light-exposed riboflavin in the presence of vitamin C. The alveolarization index correlated (r(2) = 0.35; p < 0.05) with beta-actin mRNA, suggesting alveolar disruption. Antiperoxides conferred no protection against decreased alveolarization. Lung remodeling induced by exposure of TPN to ambient light is not due to a direct effect of infused peroxides but rather to an interaction between vitamin C and peroxides generated by the exposure of riboflavin to light. It is speculated that this interaction may play a role in the development of chronic lung disease of premature infants who receive TPN and have immature antioxidant defenses.
Jean-Claude Lavoie; Thérèse Rouleau; Philippe Chessex
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-07-13
Journal Detail:
Title:  The Journal of pharmacology and experimental therapeutics     Volume:  311     ISSN:  0022-3565     ISO Abbreviation:  J. Pharmacol. Exp. Ther.     Publication Date:  2004 Nov 
Date Detail:
Created Date:  2004-10-15     Completed Date:  2005-01-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376362     Medline TA:  J Pharmacol Exp Ther     Country:  United States    
Other Details:
Languages:  eng     Pagination:  634-9     Citation Subset:  IM    
Research Centre, Sainte-Justine Hospital, 3175 Côte Ste-Catherine, Montreal, Quebec, Canada H3T 1C5.
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MeSH Terms
Ascorbic Acid / adverse effects*
Disease Models, Animal
Drug Interactions
Guinea Pigs
Lung Diseases / chemically induced*
Riboflavin / adverse effects*,  radiation effects
Reg. No./Substance:
50-81-7/Ascorbic Acid; 83-88-5/Riboflavin

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