| Interaction between antibody-diversification enzyme AID and spliceosome-associated factor CTNNBL1. | |
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MedLine Citation:
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PMID: 18722174 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Activation-induced deaminase (AID) deaminates deoxycytidine residues in immunoglobulin genes, triggering antibody diversification. Here, by use of two-hybrid and coimmunoprecipitation assays, we identify CTNNBL1 (also known as NAP) as an AID-specific interactor. Mutants of AID that interfere with CTNNBL1 interaction yield severely diminished hypermutation and class switching. Targeted inactivation of CTNNBL1 in DT40 B cells also considerably diminishes IgV diversification. CTNNBL1 is a widely expressed nuclear protein that associates with the Prp19 complex of the spliceosome, interacting with its CDC5L component. The results, therefore, identify residues in AID involved in its in vivo targeting and suggest they might act through interaction with CTNNBL1, giving possible insight into the linkage between AID recruitment and target-gene transcription. |
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Authors:
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Silvestro G Conticello; Karuna Ganesh; Kanmin Xue; Mason Lu; Cristina Rada; Michael S Neuberger |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Molecular cell Volume: 31 ISSN: 1097-4164 ISO Abbreviation: Mol. Cell Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-08-25 Completed Date: 2008-09-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9802571 Medline TA: Mol Cell Country: United States |
Other Details:
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Languages: eng Pagination: 474-84 Citation Subset: IM |
Affiliation:
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Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB20QH, UK. silvo.conticello@ittumori.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibody Diversity* Apoptosis Regulatory Proteins / metabolism* Cell Cycle Proteins / metabolism Cell Line Cell Nucleus / metabolism Chickens Cytidine Deaminase / metabolism* Humans Mutant Proteins / metabolism Mutation / genetics Nuclear Proteins / metabolism* Protein Binding RNA-Binding Proteins / metabolism Rats Spliceosomes / metabolism* Two-Hybrid System Techniques |
| Chemical | |
Reg. No./Substance:
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0/Apoptosis Regulatory Proteins; 0/CDC5L protein, human; 0/CTNNBL1 protein, human; 0/Cell Cycle Proteins; 0/Mutant Proteins; 0/Nuclear Proteins; 0/RNA-Binding Proteins; EC 3.5.4.-/AICDA (activation-induced cytidine deaminase); EC 3.5.4.5/Cytidine Deaminase |
| Comments/Corrections | |
Comment In:
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Mol Cell. 2008 Sep 5;31(5):615-6
[PMID:
18775319
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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