Document Detail


Interaction between CD44 and hyaluronate induces chemoresistance in non-small cell lung cancer cell.
MedLine Citation:
PMID:  17276588     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
CD44s is a principle hyaluronate (HA) receptor and has been reported to play an important role in cancer cell invasion and metastasis. The aim of our study is to determine if the interaction between HA and CD44s influences in vitro chemosensitivity of non-small cell lung cancer (NSCLC). NSCLC cell line, H322 cells, transfected with the CD44s gene (H322/CD44s) cultured on HA coated plates were more resistant to cisplatin (CDDP) than that on bovine serum albumin. Multidrug resistance protein2 (MRP2) expression was induced in H322/CD44s cells cultured on HA. MRP2 inhibitor, MK571, not only suppressed MRP2 expression but also reversed CDDP resistance. These results suggest that the interaction between CD44s and HA play a pivotal role in acquired resistance to CDDP in NSCLC and MRP2 could be involved in this potential mechanism.
Authors:
Rina Ohashi; Fumiyuki Takahashi; Ri Cui; Masakata Yoshioka; Tao Gu; Shinichi Sasaki; Shigeru Tominaga; Kazuto Nishio; Kenneth K Tanabe; Kazuhisa Takahashi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-02-05
Journal Detail:
Title:  Cancer letters     Volume:  252     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-05-30     Completed Date:  2007-10-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  225-34     Citation Subset:  IM    
Affiliation:
Department of Respiratory Medicine, Juntendo University, School of Medicine, 2-1-1 Hongo, Bunkyo-Ku, Tokyo 113-8421, Japan. rinaohas@med.juntendo.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD44 / metabolism*
Apoptosis
Blotting, Western
Carcinoma, Non-Small-Cell Lung / metabolism*,  pathology
Cell Adhesion
Cell Line, Tumor
Drug Resistance, Multiple*
Fluorescent Antibody Technique
Humans
Hyaluronic Acid / metabolism*
In Situ Nick-End Labeling
Lung Neoplasms / metabolism*,  pathology
Membrane Transport Proteins / metabolism
Multidrug Resistance-Associated Proteins / antagonists & inhibitors,  metabolism
Chemical
Reg. No./Substance:
0/Antigens, CD44; 0/Membrane Transport Proteins; 0/Multidrug Resistance-Associated Proteins; 0/multidrug resistance-associated protein 2; 9004-61-9/Hyaluronic Acid

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