| Interaction of aromatic nitro compounds with reduced hepatic microsomal cytochrome P-450. | |
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MedLine Citation:
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PMID: 240656 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Aromatic nitro compounds interact with sodium dithionite-reduced microsomes to generate difference spectra characterized by a maximum at 400 nm and minima at 440 and 528 nm. Spectral binding constants of approximately 1 mM were calculated for ferrohemochromes in which nitrobenzene, p-nitrobenzoate, 2-nitrofluorene, and 2-nitronaphthalene served as ligands. Nitro binding affinity was increased approximately 3-fold by pretreatment of animals with phenobarbital (KS congruent to 0.3 mM). The interaction of nitro compounds with reduced microsomes from 3-methylcholanthrene-pretreated animals, however, failed to give discernable difference spectra. There is apparent mutual inhibition of binding of carbon monoxide, metyrapone, and nitro compounds with reduced cytochrome P-450, since addition of metyrapone or carbon monoxide to reference and sample cuvettes does not alter qualitative aspects of the nitro binding spectra, but only its magnitude. From the relative KS values, it is concluded that metyrapone and carbon monoxide interact with reduced cytochrome P-450 more tenaciously than nitro compounds. |
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Authors:
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L A Sternson; R E Gammans |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Drug metabolism and disposition: the biological fate of chemicals Volume: 3 ISSN: 0090-9556 ISO Abbreviation: Drug Metab. Dispos. Publication Date: 1975 Jul-Aug |
Date Detail:
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Created Date: 1975-12-28 Completed Date: 1975-12-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9421550 Medline TA: Drug Metab Dispos Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 266-74 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aerobiosis Amines / metabolism Animals Carbon Monoxide / metabolism Cytochrome P-450 Enzyme System / metabolism* Hydroxylamines / metabolism Kinetics Male Metyrapone / metabolism Microsomes, Liver / metabolism* Nitro Compounds / metabolism* Nitroso Compounds / metabolism Rabbits Spectrophotometry |
| Chemical | |
Reg. No./Substance:
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0/Amines; 0/Hydroxylamines; 0/Nitro Compounds; 0/Nitroso Compounds; 54-36-4/Metyrapone; 630-08-0/Carbon Monoxide; 9035-51-2/Cytochrome P-450 Enzyme System |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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