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Interaction of amosite and surface-modified amosite with a V79-4 (Chinese hamster lung) cell line.
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MedLine Citation:
PMID:  2166656     Owner:  NLM     Status:  MEDLINE    
We have been examining a number of chemically modified mineral fibers, derived from amosite asbestos, by in vitro methods to clarify the role of the fiber surface in determining biological activity. The various fibers have identical size distributions but differ in their affinities for components of the cell membrane. They were treated with boiling toluene or chemically modified by treatment with alkyldimethylchlorosilanes (R = C8, C18) that react with free-surface hydroxyl groups to form the corresponding siloxanes. Fibers in MEM supplemented with 15% fetal calf serum were added to a suspension of V79-4 cells labeled with tritiated thymidine and the mixture was incubated. Aliquots of this mixture were spun down on a density gradient to determine the degree of cell-fiber interaction. At 37 degrees C native amosite (UICC standard) stuck to cells within 15 min of incubation, and the amount of sticking was maximum within 70 min. Decreasing the temperature decreased the amount of sticking, and at 20 degrees C no sticking was observable. The chemically modified amosite and the amosite treated with boiling toluene did not stick to the cells even after 70 min. Soaking the toluene-treated amosite with aqueous solutions at room temperature for 48 hr produced a material that had the same sticking properties as the original untreated fiber. These results indicate that the silanol content, and possibly the degree of hydration of the fiber surface, is important for a fiber to stick to a cell surface.
E A Sara; R C Brown; C E Evans; J A Hoskins; C F Simpson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Environmental health perspectives     Volume:  85     ISSN:  0091-6765     ISO Abbreviation:  Environ. Health Perspect.     Publication Date:  1990 Apr 
Date Detail:
Created Date:  1990-09-17     Completed Date:  1990-09-17     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0330411     Medline TA:  Environ Health Perspect     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  101-5     Citation Subset:  IM    
Medical Research Council Toxicology Unit, Carshalton, Surrey, UK.
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MeSH Terms
Asbestos / pharmacology*
Asbestos, Amosite
Cell Adhesion / drug effects*
Cell Line
Chemical Phenomena
Lung / cytology*
Silicon / pharmacology*
Surface Properties*
Toluene / pharmacology
Trimethylsilyl Compounds / pharmacology*
Reg. No./Substance:
0/Trimethylsilyl Compounds; 108-88-3/Toluene; 12172-73-5/Asbestos, Amosite; 1332-21-4/Asbestos; 7440-21-3/Silicon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): Environ Health Perspect
ISSN: 0091-6765
Article Information
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Print publication date: Month: 4 Year: 1990
Volume: 85First Page: 101 Last Page: 105
ID: 1568335
PubMed Id: 2166656

Interaction of amosite and surface-modified amosite with a V79-4 (Chinese hamster lung) cell line.
E A Sara
R C Brown
C E Evans
J A Hoskins
C F Simpson
Medical Research Council Toxicology Unit, Carshalton, Surrey, UK.

Article Categories:
  • Research Article

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