Document Detail

Interaction of zinc, ascorbic acid, and folic acid in glycation with albumin as protein model.
MedLine Citation:
PMID:  20143181     Owner:  NLM     Status:  MEDLINE    
Using albumin as model, we conducted series of in vitro glycation experiments to examine role of zinc in glycation using glucose at 4-100 mg/ml, incubations at 37°C or 60°C, duration of 2 or 4 weeks and in presence of zinc or ascorbic acid (AA) or folic acid (FA). Modifications of bovine serum albumin (BSA) were examined by using fluorescence of advanced glycation end products (AGEs) and dityrosine, UV, and Fourier transformed infrared spectroscopy. Adding zinc (0 to 768.5 μmol/l) resulted in significant inhibition of albumin glycation by glucose with a linear fit, y = -0·0895x + 230·99(R² = 0·7676, p = 0·013). The glycation by fructose was greater than that of glucose with stronger inhibitory effect by zinc in fructose-glycation (t= -5.8, p=0.002). Addition of zinc significantly decreased fluorescence as seen in Zn + FA or Zn + AA sets as compared to sets of FA alone (p=0.00056) or AA alone (p=0.037). The fluorescence for dityrosine and AGE had a correlation of 0.897 (p<0.01). The data from fluorescence, UV, and FTIR spectra collectively suggested inhibitory effect of zinc in BSA glycation alone or in presence of FA and AA, showing new dimension for the protective action of zinc in hyperglycemic conditions.
Rashmi Tupe; Vaishali Agte
Publication Detail:
Type:  Journal Article     Date:  2010-02-09
Journal Detail:
Title:  Biological trace element research     Volume:  138     ISSN:  1559-0720     ISO Abbreviation:  Biol Trace Elem Res     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-08     Completed Date:  2011-02-24     Revised Date:  2011-03-11    
Medline Journal Info:
Nlm Unique ID:  7911509     Medline TA:  Biol Trace Elem Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  346-57     Citation Subset:  IM    
Agharkar Research Institute, G.G. Agarkar Road, Pune 411004, India.
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MeSH Terms
Ascorbic Acid / chemistry*
Folic Acid / chemistry*
Glycosylation End Products, Advanced / chemistry*
Spectroscopy, Fourier Transform Infrared
Tyrosine / analogs & derivatives*,  chemistry
Zinc / chemistry*
Reg. No./Substance:
0/Glycosylation End Products, Advanced; 50-81-7/Ascorbic Acid; 55520-40-6/Tyrosine; 59-30-3/Folic Acid; 7440-66-6/Zinc; 980-21-2/dityrosine

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