| Interaction of Rho-kinase with myosin II at stress fibres. | |
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MedLine Citation:
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PMID: 15265008 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rho-kinase and myosin phosphatase cooperatively regulate the phosphorylation level of myosin light chain and are involved in the formation of stress fibres and smooth muscle contraction. Rho-kinase has been known to be localized at stress fibres, but little is known about the mechanism of its localization. Here we identified non-muscle myosin heavy chain IIA and IIB as the pleckstrin homology domain-interacting molecules by affinity column chromatography. The pleckstrin homology domain of Rho-kinase binds to myosin II directly in in vitro cosedimentation assay. The C-terminal region of the pleckstrin homology domain was important for this interaction, and the point mutations in the pleckstrin homology domain mutant (W1170A, W1340L) resulted in a decrease in the binding. We also found that the pleckstrin homology domain, but not the pleckstrin homology domain mutant (W1170A, W1340L), was localized at stress fibres in fibroblasts. These results indicate that Rho-kinase is localized at stress fibres through binding of the pleckstrin homology domain to myosin II. |
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Authors:
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Saeko Kawabata; Jiro Usukura; Nobuhiro Morone; Masaaki Ito; Akihiro Iwamatsu; Kozo Kaibuchi; Mutsuki Amano |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Genes to cells : devoted to molecular & cellular mechanisms Volume: 9 ISSN: 1356-9597 ISO Abbreviation: Genes Cells Publication Date: 2004 Jul |
Date Detail:
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Created Date: 2004-07-21 Completed Date: 2005-02-23 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9607379 Medline TA: Genes Cells Country: England |
Other Details:
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Languages: eng Pagination: 653-60 Citation Subset: IM |
Affiliation:
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Department of Cell Pharmacology, Graduate School of Medicine, Nagoya University, Nagoya, Aichi 466-8550, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Chromatography, Affinity DNA Primers Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique Intracellular Signaling Peptides and Proteins Mice Microscopy, Electron Myosin Type II / metabolism* NIH 3T3 Cells Protein Binding Protein-Serine-Threonine Kinases / metabolism* rho-Associated Kinases |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/Intracellular Signaling Peptides and Proteins; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/rho-Associated Kinases; EC 3.6.1.-/Myosin Type II |
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