Document Detail

Interaction Of The Rattlesnake Toxin Crotamine With Model Membranes.
MedLine Citation:
PMID:  24754574     Owner:  NLM     Status:  Publisher    
Crotamine is one of the main constituents of the venom of the South American rattlesnake Crotalus durissus terrificus. A common gene ancestry and structural similarity with the antimicrobial β-defensins (identical disulfide bond pattern and highly positive net charge) suggested potential antimicrobial activities for this snake toxin. Although crotamine demonstrated a faint activity against both Gram-positive and Gram-negative bacteria, a pronounced antifungal activity was observed against Candida spp., Trichosporon spp., and Cryptococcus neoformans. Crotamine's selective antimicrobial property, with no observable hemolytic activity, stimulated us to evaluate the potential applications of this polypeptide as an anti-yeast or candicidal agent for medical and industrial application. Aiming to understand the mechanism(s) of action underlying crotamine antimicrobial activity and its selectivity for fungi, we present herein further studies using membrane model systems (i.e. large unilamellar vesicles, LUVs, and giant unilamellar vesicles, GUVs), with different phospholipid compositions. We show here that crotamine presents a higher lytic activity on negatively charged membranes compared with neutral charged membranes with neutral charge, with or without cholesterol or ergosterol content. The vesicle burst was not preceded by membrane permeabilization as it is generally observed for pore forming peptides. Although such a property of disrupting lipid membranes is very important to combat multi-resistant fungi, no inhibitory activity was observed for crotamine against biofilms formed by several Candida spp. strains, except for a limited effect against C. krusei biofilm.
Bruno Andrade Costa; Leonardo Sanches; Andreza Gomide; Fernando Bizerra; Caroline Dal Mas; Eduardo Brandt Oliveira; Katia Regina Perez; Rosangela Itri; Nancy Oguiura; Mirian A F Hayashi
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-4-23
Journal Detail:
Title:  The journal of physical chemistry. B     Volume:  -     ISSN:  1520-5207     ISO Abbreviation:  J Phys Chem B     Publication Date:  2014 Apr 
Date Detail:
Created Date:  2014-4-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101157530     Medline TA:  J Phys Chem B     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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