Document Detail


Interaction of Rab3B with microtubule-binding protein Gas8 in NIH 3T3 cells.
MedLine Citation:
PMID:  18396146     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rab3 subfamily small G proteins (Rab3A, Rab3B, Rab3C, and Rab3D) control the regulated exocytosis in neuronal/secretory cells. Rab3B is also detected and upregulated in non-neuronal/non-secretory cells, whereas its function remains elusive. In the present study, we identified growth-arrest-specific gene 8 (Gas8), an evolutionally conserved microtubule-binding protein that is upregulated in growth-arrested NIH 3T3 cells and involved in the dynein motor regulation in flagellar/ciliary axoneme, as a novel Rab3B-binding protein using a yeast two-hybrid system. Rab3B as well as Gas8 was upregulated in growth-arrested NIH 3T3 cells and enriched in testis and lung with well-developed flagella/cilia. Gas8 was specifically interacted with the GTP-bound form of Rab3B and co-localized with Rab3B at the Golgi in NIH 3T3 cells. Furthermore, Rab3B was relocated upon expression of the Rab3B-binding domain of Gas8. These results suggest that Gas8 links Rab3B to microtubules in NIH 3T3 cells.
Authors:
Noriyuki Nishimura; Kunihiko Araki; Wakako Shinahara; Yumiko Nakano; Kaho Nishimura; Hironori Higashio; Takuya Sasaki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-31
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  474     ISSN:  1096-0384     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-27     Completed Date:  2008-08-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  United States    
Other Details:
Languages:  eng     Pagination:  136-42     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
Cricetinae
Immunoprecipitation
Mice
Microscopy, Fluorescence
NIH 3T3 Cells
Protein Binding
Proteins / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Two-Hybrid System Techniques
rab3 GTP-Binding Proteins / chemistry,  metabolism*
Chemical
Reg. No./Substance:
0/Gas8 protein, mouse; 0/Proteins; EC 3.6.1.-/Rab3b protein, mouse; EC 3.6.5.2/rab3 GTP-Binding Proteins

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