| Interaction of Rab3B with microtubule-binding protein Gas8 in NIH 3T3 cells. | |
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MedLine Citation:
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PMID: 18396146 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rab3 subfamily small G proteins (Rab3A, Rab3B, Rab3C, and Rab3D) control the regulated exocytosis in neuronal/secretory cells. Rab3B is also detected and upregulated in non-neuronal/non-secretory cells, whereas its function remains elusive. In the present study, we identified growth-arrest-specific gene 8 (Gas8), an evolutionally conserved microtubule-binding protein that is upregulated in growth-arrested NIH 3T3 cells and involved in the dynein motor regulation in flagellar/ciliary axoneme, as a novel Rab3B-binding protein using a yeast two-hybrid system. Rab3B as well as Gas8 was upregulated in growth-arrested NIH 3T3 cells and enriched in testis and lung with well-developed flagella/cilia. Gas8 was specifically interacted with the GTP-bound form of Rab3B and co-localized with Rab3B at the Golgi in NIH 3T3 cells. Furthermore, Rab3B was relocated upon expression of the Rab3B-binding domain of Gas8. These results suggest that Gas8 links Rab3B to microtubules in NIH 3T3 cells. |
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Authors:
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Noriyuki Nishimura; Kunihiko Araki; Wakako Shinahara; Yumiko Nakano; Kaho Nishimura; Hironori Higashio; Takuya Sasaki |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-03-31 |
Journal Detail:
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Title: Archives of biochemistry and biophysics Volume: 474 ISSN: 1096-0384 ISO Abbreviation: Arch. Biochem. Biophys. Publication Date: 2008 Jun |
Date Detail:
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Created Date: 2008-05-27 Completed Date: 2008-08-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: United States |
Other Details:
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Languages: eng Pagination: 136-42 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western Cricetinae Immunoprecipitation Mice Microscopy, Fluorescence NIH 3T3 Cells Protein Binding Proteins / metabolism* Reverse Transcriptase Polymerase Chain Reaction Two-Hybrid System Techniques rab3 GTP-Binding Proteins / chemistry, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Gas8 protein, mouse; 0/Proteins; EC 3.6.1.-/Rab3b protein, mouse; EC 3.6.5.2/rab3 GTP-Binding Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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