| Interaction of P-glycoprotein with a hydrophobic component of rat urine. | |
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MedLine Citation:
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PMID: 1353959 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The presence of an endogenous P-glycoprotein substrate in rat urine was examined by testing the ability of a hydrophobic extract to reverse multidrug resistance in CHO cells and to inhibit [3H]azidopine photolabelling. The accumulation of several hydrophobic drugs and dyes, known to be transported by P-glycoprotein, was dramatically enhanced in multidrug-resistant CHO cells (CHRC5) by a component contained in a hydrophobic extract prepared from rat urine by octadecyl (C18) reverse phase chromatography. The biological action of this urinary component involves a direct interaction with P-glycoprotein since it blocked photolabelling of the protein with [3H]azidopine. The effective concentration of the substance required to enhance drug accumulation and inhibit photolabelling was similar and within the range of its urinary content. These results suggest that a hydrophobic substance in urine may be an endogenous substrate of kidney P-glycoprotein. |
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Authors:
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J H Charuk; R A Reithmeier |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 186 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1992 Jul |
Date Detail:
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Created Date: 1992-09-04 Completed Date: 1992-09-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 796-802 Citation Subset: IM |
Affiliation:
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Department of Medicine, University of Toronto, Ontario, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Azides / metabolism CHO Cells Cricetinae Dihydropyridines / metabolism Drug Resistance / physiology* Female Kinetics Male Membrane Glycoproteins / antagonists & inhibitors, metabolism* P-Glycoprotein Rats Tissue Extracts / pharmacology* Urine / physiology* Verapamil / pharmacology Vinblastine / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Azides; 0/Dihydropyridines; 0/Membrane Glycoproteins; 0/P-Glycoprotein; 0/Tissue Extracts; 52-53-9/Verapamil; 865-21-4/Vinblastine; 90523-31-2/azidopine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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