Document Detail


Interaction of nucleic acids with the glycocalyx.
MedLine Citation:
PMID:  22400897     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mammalian cells resist the uptake of nucleic acids. The lipid bilayer of the plasma membrane presents one barrier. Here, we report on a second physicochemical barrier for uptake. To create a sensitive probe for nucleic acid-cell interactions, we synthesized fluorescent conjugates in which lipids are linked to DNA oligonucleotides. We found that these conjugates incorporate readily into the plasma membrane but are not retained there. Expulsion of lipid-oligonucleotide conjugates from the plasma membrane increases with oligonucleotide length. Conversely, the incorporation of conjugates increases markedly in cells that lack the major anionic components of the glycocalyx, sialic acid and glycosaminoglycans, and in cells that had incorporated highly cationic lipids into their plasma membrane. We conclude that anionic oligosaccharides provide a formidable barrier to the uptake of nucleic acids by mammalian cells. This conclusion has implications for genomic stability, as well as the delivery of genes and siRNAs into mammalian cells.
Authors:
Michael J Palte; Ronald T Raines
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-03-29
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  134     ISSN:  1520-5126     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-04-11     Completed Date:  2012-08-16     Revised Date:  2014-11-13    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6218-23     Citation Subset:  IM    
Copyright Information:
© 2012 American Chemical Society
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MeSH Terms
Descriptor/Qualifier:
Animals
Anions
Cations
DNA / chemistry
Glycocalyx / chemistry*
Glycosaminoglycans / chemistry
HeLa Cells
Humans
Lipid Bilayers / chemistry
Lipids / chemistry
Models, Chemical
Models, Molecular
Molecular Conformation
N-Acetylneuraminic Acid / chemistry
Nucleic Acids / chemistry*
Oligonucleotides / chemistry
Oligosaccharides / chemistry
RNA, Small Interfering / metabolism
Grant Support
ID/Acronym/Agency:
R01 CA073808/CA/NCI NIH HHS; R01 CA073808/CA/NCI NIH HHS; R01 CA073808-16/CA/NCI NIH HHS; R01 GM044783/GM/NIGMS NIH HHS; R01 GM044783/GM/NIGMS NIH HHS; R01 GM044783-21/GM/NIGMS NIH HHS; T32 GM008688/GM/NIGMS NIH HHS; T32 GM008688/GM/NIGMS NIH HHS; T32 GM008688-15/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Anions; 0/Cations; 0/Glycosaminoglycans; 0/Lipid Bilayers; 0/Lipids; 0/Nucleic Acids; 0/Oligonucleotides; 0/Oligosaccharides; 0/RNA, Small Interfering; 9007-49-2/DNA; GZP2782OP0/N-Acetylneuraminic Acid
Comments/Corrections

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