| Interaction of nucleic acids with the glycocalyx. | |
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MedLine Citation:
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PMID: 22400897 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mammalian cells resist the uptake of nucleic acids. The lipid bilayer of the plasma membrane presents one barrier. Here, we report on a second physicochemical barrier for uptake. To create a sensitive probe for nucleic acid-cell interactions, we synthesized fluorescent conjugates in which lipids are linked to DNA oligonucleotides. We found that these conjugates incorporate readily into the plasma membrane but are not retained there. Expulsion of lipid-oligonucleotide conjugates from the plasma membrane increases with oligonucleotide length. Conversely, the incorporation of conjugates increases markedly in cells that lack the major anionic components of the glycocalyx, sialic acid and glycosaminoglycans, and in cells that had incorporated highly cationic lipids into their plasma membrane. We conclude that anionic oligosaccharides provide a formidable barrier to the uptake of nucleic acids by mammalian cells. This conclusion has implications for genomic stability, as well as the delivery of genes and siRNAs into mammalian cells. |
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Authors:
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Michael J Palte; Ronald T Raines |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-03-29 |
Journal Detail:
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Title: Journal of the American Chemical Society Volume: 134 ISSN: 1520-5126 ISO Abbreviation: J. Am. Chem. Soc. Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-04-11 Completed Date: 2012-08-16 Revised Date: 2013-05-20 |
Medline Journal Info:
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Nlm Unique ID: 7503056 Medline TA: J Am Chem Soc Country: United States |
Other Details:
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Languages: eng Pagination: 6218-23 Citation Subset: IM |
Copyright Information:
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© 2012 American Chemical Society |
Affiliation:
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Medical Scientist Training Program, Molecular & Cellular Pharmacology Graduate Training Program, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anions Cations DNA / chemistry Glycocalyx / chemistry* Glycosaminoglycans / chemistry HeLa Cells Humans Lipid Bilayers / chemistry Lipids / chemistry Models, Chemical Models, Molecular Molecular Conformation N-Acetylneuraminic Acid / chemistry Nucleic Acids / chemistry* Oligonucleotides / chemistry Oligosaccharides / chemistry RNA, Small Interfering / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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R01 CA073808/CA/NCI NIH HHS; R01 CA073808/CA/NCI NIH HHS; R01 CA073808-16/CA/NCI NIH HHS; R01 GM044783/GM/NIGMS NIH HHS; R01 GM044783-21/GM/NIGMS NIH HHS; T32 GM008688/GM/NIGMS NIH HHS; T32 GM008688-15/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Anions; 0/Cations; 0/Glycosaminoglycans; 0/Lipid Bilayers; 0/Lipids; 0/Nucleic Acids; 0/Oligonucleotides; 0/Oligosaccharides; 0/RNA, Small Interfering; 131-48-6/N-Acetylneuraminic Acid; 9007-49-2/DNA |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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