| Interaction of D-amino acid oxidase with carbon nanotubes: implications in the design of biosensors. | |
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MedLine Citation:
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PMID: 19132842 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have investigated the interaction of d-amino acid oxidase (DAAO) with single-walled carbon nanotubes (CNT) by spectroscopic ellipsometry. Dynamic adsorption experiments were performed at different experimental conditions. In addition, the activity of the enzyme adsorbed at different conditions was studied. Our results indicate that DAAO can be adsorbed to CNT at different pH values and concentrations by a combination of hydrophobic and electrostatic interactions. Considering that the highest enzymatic activity was obtained by adsorbing the protein at pH 5.7 and 0.1 mg x mL(-1), our results indicate that DAAO can adopt multiple orientations on the surface, which are ultimately responsible for significant differences in catalytic activity. |
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Authors:
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Maria F Mora; Carla E Giacomelli; Carlos D Garcia |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Analytical chemistry Volume: 81 ISSN: 1520-6882 ISO Abbreviation: Anal. Chem. Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2009-01-30 Completed Date: 2009-03-04 Revised Date: 2010-09-22 |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: United States |
Other Details:
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Languages: eng Pagination: 1016-22 Citation Subset: IM |
Affiliation:
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Department of Chemistry, The University of Texas at San Antonio, San Antonio, Texas 78249, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biocatalysis Biosensing Techniques / methods* D-Amino-Acid Oxidase / chemistry, metabolism* Hydrogen-Ion Concentration Kinetics Models, Chemical Nanotubes, Carbon / chemistry* |
| Grant Support | |
ID/Acronym/Agency:
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1SC3GM081085/GM/NIGMS NIH HHS; SC3 GM081085-01/GM/NIGMS NIH HHS; SC3 GM081085-02/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Nanotubes, Carbon; EC 1.4.3.3/D-Amino-Acid Oxidase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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