| Interaction of cell turgor and hormones on sucrose uptake in isolated Phloem of celery. | |
| | |
MedLine Citation:
|
PMID: 16665557 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
|
Phloem tissue isolated from celery (Apium graveolens L.) was used to investigate the regulation of sucrose uptake by turgor (manipulated by 50-400 milliosomolal solutions of polyethylene glycol) and hormones indoleacetic acid (IAA) and gibberillic acid (GA(3)). Sucrose uptake was enhanced under low cellular turgor (increase in the V(max)). Furthermore, enhancement of sucrose uptake was due to a net increase in influx rates since sucrose efflux was not affected by cell turgor. Manipulations of cell turgor had no effect on 3-O-methyl glucose uptake. When 20 millimolar buffer was present in uptake solutions, low turgor-induced effects were observed only at low pH range (4.5-5.5). However, the effect was extended to higher external pH (up to 7.5) when buffer was omitted from uptake solutions. A novel interaction between cellular turgor and hormone treatments was observed, in that GA(3) (10 micromolar) and IAA (0.1-100 micromolar) enhanced sucrose uptake only at moderate turgor levels. The hormones elicited little or no response on sucrose uptake under conditions of low or high cell turgor. Low cell turgor, IAA, GA(3), and fusicoccin caused acidification by isolated phloem segments in a buffer-free solution. It is suggested that enhanced sucrose uptake in response to low turgor and/or hormones was mediated through the plasmalemma H(+)-ATPase and most likely occurred at the site of loading. |
| | |
Authors:
|
J Daie |
Related Documents
:
|
22549337 - Effect of porcine placenta steroid extract on myogenic satellite cell proliferation, tr... 557367 - Mechanism of uptake of nitrosoureas by l5178y lymphoblasts in vitro. 2820507 - Intracellular chromium reduction. 20079367 - Protein kinase c isotypes in signal transduction for the 1,25d3-marrs receptor (erp57/p... 22246987 - The enhanced anti-cancer effect of hexenyl ester of 5-aminolaevulinic acid photodynamic... 7501297 - A novel factor, ta20, involved in neuronal differentiation: cdna cloning and expression. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Plant physiology Volume: 84 ISSN: 0032-0889 ISO Abbreviation: Plant Physiol. Publication Date: 1987 Aug |
Date Detail:
|
Created Date: 2010-06-29 Completed Date: 2010-06-29 Revised Date: 2010-09-14 |
Medline Journal Info:
|
Nlm Unique ID: 0401224 Medline TA: Plant Physiol Country: United States |
Other Details:
|
Languages: eng Pagination: 1033-7 Citation Subset: - |
Affiliation:
|
Department of Soils and Crops, Cook College-Rutgers University, New Brunswick, New Jersey 08903. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Immunocytochemical Localization of Phosphoribulose Kinase in the Cyanelles of Cyanophora paradoxa an...
Next Document: Identification of Polysaccharide Hydrolases Involved in Autolytic Degradation of Zea Cell Walls.