Document Detail


Interaction of Beclin 1 with survivin regulates sensitivity of human glioma cells to TRAIL-induced apoptosis.
MedLine Citation:
PMID:  20638385     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We reported a novel interaction between Beclin 1, a key regulator of autophagy, and survivin, a member of the inhibitor of apoptosis protein family. We found that knock-down of Beclin 1 down-regulated survivin protein, and the turnover rate of survivin was increased when Beclin 1 expression was silenced. Knock-down of Beclin 1 sensitized glioma cells to TRAIL-induced apoptosis, and introduction of survivin antagonized the sensitizing effect, suggesting that down-regulation of survivin mediates the enhanced sensitivity to TRAIL-induced apoptosis. These results demonstrate a novel interaction between Beclin 1 and survivin, and may provide a potential mechanism underlying the cross-talk between autophagy and apoptosis.
Authors:
Ting-Kuang Niu; Yan Cheng; Xingcong Ren; Jin-Ming Yang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-07-16
Journal Detail:
Title:  FEBS letters     Volume:  584     ISSN:  1873-3468     ISO Abbreviation:  FEBS Lett.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-16     Completed Date:  2010-09-03     Revised Date:  2011-11-14    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  3519-24     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Affiliation:
Department of Pharmacology and The Penn State Cancer Institute, The Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center, 500 University Drive, Hershey, PA 17033, USA.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects,  physiology*
Apoptosis Regulatory Proteins / antagonists & inhibitors,  genetics,  metabolism*
Base Sequence
Cell Line, Tumor
DNA Primers / genetics
Down-Regulation
Gene Knockdown Techniques
Glioma / drug therapy,  metabolism*,  pathology*
Humans
Membrane Proteins / antagonists & inhibitors,  genetics,  metabolism*
Microtubule-Associated Proteins / genetics,  metabolism*
Protein Interaction Mapping
RNA, Small Interfering / genetics
Recombinant Proteins / metabolism,  pharmacology
TNF-Related Apoptosis-Inducing Ligand / metabolism*,  pharmacology
Grant Support
ID/Acronym/Agency:
R01 CA066077-12/CA/NCI NIH HHS; R01 CA135038-01A2/CA/NCI NIH HHS; R01 CA135038-02/CA/NCI NIH HHS; R01 CA135038-03/CA/NCI NIH HHS; R01CA 135038/CA/NCI NIH HHS; R01CA 66077/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Apoptosis Regulatory Proteins; 0/BECN1 protein, human; 0/BIRC5 protein, human; 0/DNA Primers; 0/Membrane Proteins; 0/Microtubule-Associated Proteins; 0/RNA, Small Interfering; 0/Recombinant Proteins; 0/TNF-Related Apoptosis-Inducing Ligand; 0/TNFSF10 protein, human

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