Document Detail

Intensity and anisotropy decays of the Wye base of yeast tRNA(Phe) as measured by frequency-domain fluorometry.
MedLine Citation:
PMID:  3647800     Owner:  NLM     Status:  MEDLINE    
The intensity and anisotropy decays of Wye base fluorescence from yeast tRNA(Phe) were determined by frequency-domain fluorometry. The intensity decay is at least a double exponential in the presence and absence of Mg2+, but the multi-exponential character of the decay is more pronounced in the absence of Mg2+. The anisotropy decay displays components due to overall tRNA rotational diffusion and to local torsional motions. The amplitude of the local motion is decreased 2-fold by the presence of Mg2+. The results are broadly consistent with a more homogeneous environment for the Wye base in the presence of Mg2+.
B D Wells; J R Lakowicz
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biophysical chemistry     Volume:  26     ISSN:  0301-4622     ISO Abbreviation:  Biophys. Chem.     Publication Date:  1987 Apr 
Date Detail:
Created Date:  1987-07-29     Completed Date:  1987-07-29     Revised Date:  2012-08-01    
Medline Journal Info:
Nlm Unique ID:  0403171     Medline TA:  Biophys Chem     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  39-43     Citation Subset:  IM    
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MeSH Terms
Chelating Agents
Guanine / analogs & derivatives*,  analysis
RNA, Transfer, Amino Acyl / analysis*
Spectrometry, Fluorescence
Yeasts / analysis
Grant Support
Reg. No./Substance:
0/Chelating Agents; 0/RNA, Transfer, Amino Acyl; 33359-03-4/wye; 73-40-5/Guanine; 7439-95-4/Magnesium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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