Document Detail


Intensity and anisotropy decays of the Wye base of yeast tRNA(Phe) as measured by frequency-domain fluorometry.
MedLine Citation:
PMID:  3647800     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The intensity and anisotropy decays of Wye base fluorescence from yeast tRNA(Phe) were determined by frequency-domain fluorometry. The intensity decay is at least a double exponential in the presence and absence of Mg2+, but the multi-exponential character of the decay is more pronounced in the absence of Mg2+. The anisotropy decay displays components due to overall tRNA rotational diffusion and to local torsional motions. The amplitude of the local motion is decreased 2-fold by the presence of Mg2+. The results are broadly consistent with a more homogeneous environment for the Wye base in the presence of Mg2+.
Authors:
B D Wells; J R Lakowicz
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biophysical chemistry     Volume:  26     ISSN:  0301-4622     ISO Abbreviation:  Biophys. Chem.     Publication Date:  1987 Apr 
Date Detail:
Created Date:  1987-07-29     Completed Date:  1987-07-29     Revised Date:  2012-08-01    
Medline Journal Info:
Nlm Unique ID:  0403171     Medline TA:  Biophys Chem     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  39-43     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Chelating Agents
Guanine / analogs & derivatives*,  analysis
Magnesium
RNA, Transfer, Amino Acyl / analysis*
Spectrometry, Fluorescence
Thermodynamics
Yeasts / analysis
Grant Support
ID/Acronym/Agency:
GM 30700/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Chelating Agents; 0/RNA, Transfer, Amino Acyl; 33359-03-4/wye; 73-40-5/Guanine; 7439-95-4/Magnesium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Hemodynamic and ventilatory effects of high-frequency jet and conventional ventilation in piglets wi...
Next Document:  The Vancouver Lymphadenopathy-AIDS Study: 7. Clinical and laboratory features of 87 cases of primary...