| Intense correlation between brain infarction and protein-conjugated acrolein. | |
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MedLine Citation:
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PMID: 19661476 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND AND PURPOSE: We recently found that increases in plasma levels of protein-conjugated acrolein and polyamine oxidases, enzymes that produce acrolein, are good markers for stroke. The aim of this study was to determine whether the level of protein-conjugated acrolein is increased and levels of spermine and spermidine, the substrates of acrolein production, are decreased at the locus of infarction. METHODS: A unilateral infarction was induced in mouse brain by photoinduction after injection of Rose Bengal. The volume of the infarction was analyzed using the public domain National Institutes of Health image program. The level of protein-conjugated acrolein at the locus of infarction and in plasma was measured by Western blotting and enzyme-linked immunosorbent assay, respectively. The levels of polyamines at the locus of infarction and in plasma were measured by high-performance liquid chromatography. RESULTS: The level of protein-conjugated acrolein was greatly increased, and levels of spermine and spermidine were decreased at the locus of infarction at 24 hours after the induction of stroke. The size of infarction was significantly decreased by N-acetylcysteine, a scavenger of acrolein. It was also found that the increases in the protein-conjugated acrolein, polyamines, and polyamine oxidases in plasma were observed after the induction of stroke. CONCLUSIONS: The results indicate that the induction of infarction is well correlated with the increase in protein-conjugated acrolein at the locus of infarction and in plasma. |
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Authors:
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Ryotaro Saiki; Kazuhiro Nishimura; Itsuko Ishii; Tomohiro Omura; Shigeru Okuyama; Keiko Kashiwagi; Kazuei Igarashi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-08-06 |
Journal Detail:
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Title: Stroke; a journal of cerebral circulation Volume: 40 ISSN: 1524-4628 ISO Abbreviation: Stroke Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-09-28 Completed Date: 2009-11-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0235266 Medline TA: Stroke Country: United States |
Other Details:
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Languages: eng Pagination: 3356-61 Citation Subset: IM |
Affiliation:
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Graduate School of Pharmaceutical Sciences, Chiba University, Chiba, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcysteine
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pharmacology,
therapeutic use Acrolein / analysis, blood* Animals Brain / metabolism, pathology, physiopathology Carrier Proteins / analysis, blood Disease Models, Animal Down-Regulation / physiology Free Radical Scavengers / pharmacology, therapeutic use Infarction, Middle Cerebral Artery / blood*, pathology, physiopathology Male Mice Mice, Inbred C57BL Photochemistry / methods Rose Bengal / radiation effects Spermidine / analysis, blood* Spermine / analysis, blood* Time Factors Up-Regulation / physiology |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Free Radical Scavengers; 107-02-8/Acrolein; 11121-48-5/Rose Bengal; 124-20-9/Spermidine; 616-91-1/Acetylcysteine; 71-44-3/Spermine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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