Document Detail

Intense pulsed light protects fibroblasts against the senescence induced by 8-methoxypsoralen plus ultraviolet-A irradiation.
MedLine Citation:
PMID:  21595550     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: The purpose of this study was to evaluate the influence of intense pulsed light (IPL) irradiation on 8-methoxypsoralen plus ultraviolet-A irradiation (PUVA)-induced senescence of fibroblasts in vitro.
BACKGROUND DATA: Exposure to PUVA may result in stress-induced senescence in fibroblasts. IPL has been widely used to treat photo-aged skin, but the mechanism is not clear.
METHODS: The expression of senescence-associated β-galactosidase (SA-β-gal) was determined by histochemical staining, cell viability was assessed via an MTT assay, telomere length was determined by real-time polymerase chain reaction (PCR), and changes in the generation of reactive oxygen species (ROS) were determined by flow cytometry (FCM).
RESULTS: In comparison with the control cells, cells irradiated with PUVA and PUVA+IPL showed a general elevation in SA-β-gal activity (p<0.05). The number of SA-β-gal-positive fibroblasts in the PUVA+IPL group was clearly lower than that in the PUVA group (p<0.05). Cell viability showed a time-dependent decrease in both the PUVA and PUVA+IPL groups, in comparison with the viability in the control group, and there was no difference in viability between the PUVA and PUVA+IPL groups. PUVA treatment shortened telomere length and increased the level of ROS, in comparison with the corresponding findings for the control cells (p<0.05), whereas irradiation with IPL after PUVA exposure prevented telomere shortening and decreased the ROS level, in comparison with PUVA treatment only (p<0.05).
CONCLUSIONS: PUVA treatment induced fibroblast senescence, and irradiation with IPL after PUVA exposure partially rejuvenated the cells, demonstrating a protective effect against PUVA-induced fibroblast senescence.
Ruiyan Wang; Wei Liu; Weijie Gu; Ping Zhang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-05-19
Journal Detail:
Title:  Photomedicine and laser surgery     Volume:  29     ISSN:  1557-8550     ISO Abbreviation:  Photomed Laser Surg     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-05     Completed Date:  2012-02-24     Revised Date:  2014-07-30    
Medline Journal Info:
Nlm Unique ID:  101222340     Medline TA:  Photomed Laser Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  685-90     Citation Subset:  IM    
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MeSH Terms
Analysis of Variance
Cell Aging / drug effects*,  radiation effects*
Cell Survival / drug effects,  radiation effects
Fibroblasts / radiation effects*
Flow Cytometry
Methoxsalen / pharmacology
Oxidative Stress
PUVA Therapy / methods
Phototherapy / methods*
Real-Time Polymerase Chain Reaction
beta-Galactosidase / metabolism
Reg. No./Substance:
EC; U4VJ29L7BQ/Methoxsalen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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