| Integrity rate of pronuclei after cryopreservation of pronuclear-zygotes as a criteria for subsequent embryo development and pregnancy. | |
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MedLine Citation:
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PMID: 18238906 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The aim of this study was to examine whether the integrity rate of pronuclei, after cryopreservation of pronuclear zygotes, could be a predictor of future embryo development and implantation. METHODS: Two-pronuclei stage zygotes (n = 862) were cryopreserved by aseptic rapid freezing in 15% ethylene glycol + 15% DMSO + 0.2 M sucrose with a 4-step exposure in 12% (v:v), 25, 50 and 100% rapid freezing solution for 2, 1, 1 min and 30-50 s, respectively, at room temperature, and then plunged into liquid nitrogen. Zygotes were rapidly warmed at a speed of 30,000 degrees C/min and subsequently expelled into a graded series of sucrose solutions (1.0, 0.5, 0.25, 0.12 and 0.06 M) at 2.5 min intervals and in vitro cultured for 5 days. If embryos had developed to blastocysts on the 5th day, they were transferred to the recipients. The rest of the embryos were fixed for evaluation of chromatin. RESULTS: Zygote development, up to the expanded blastocyst stage, after in vitro culture, was 40%, if the integrity rate of pronuclei was high, and 4% if the integrity rate was low (P < 0.05). The pregnancy rate after transfer of the 5-day blastocysts depended on the pronuclear integrity rate: 43% (125 pregnancies aftertransfer of 291 blastocysts) for those with a high integrity, and only 1 in 5 (20%) for the few blastocysts which had shown a pronuclear low integrity rate (P < 0.05). CONCLUSIONS: Integrity rate of pronuclei after cryopreservation of pronuclear zygotes was a predictor of future embryo development and implantation: high integrity rate resulted in high pregnancy rate, while zygotes with low integrity rate of pronuclei after cryopreservation had low developmental potential. |
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Authors:
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Vladimir Isachenko; Plamen Todorov; Yosif Dimitrov; Evgenia Isachenko |
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Publication Detail:
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Type: In Vitro; Journal Article Date: 2008-01-31 |
Journal Detail:
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Title: Human reproduction (Oxford, England) Volume: 23 ISSN: 1460-2350 ISO Abbreviation: Hum. Reprod. Publication Date: 2008 Apr |
Date Detail:
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Created Date: 2008-03-19 Completed Date: 2008-05-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8701199 Medline TA: Hum Reprod Country: England |
Other Details:
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Languages: eng Pagination: 819-26 Citation Subset: IM |
Affiliation:
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Institute of Biology and Immunology of Reproduction, Sofia, Bulgaria. v.isachenko@yahoo.com |
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Blastocyst / physiology* Cryopreservation* Embryo Implantation / physiology* Embryonic Development* Female Forecasting Humans Male Pregnancy Zygote / growth & development* |
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