Document Detail

Integrity rate of pronuclei after cryopreservation of pronuclear-zygotes as a criteria for subsequent embryo development and pregnancy.
MedLine Citation:
PMID:  18238906     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The aim of this study was to examine whether the integrity rate of pronuclei, after cryopreservation of pronuclear zygotes, could be a predictor of future embryo development and implantation. METHODS: Two-pronuclei stage zygotes (n = 862) were cryopreserved by aseptic rapid freezing in 15% ethylene glycol + 15% DMSO + 0.2 M sucrose with a 4-step exposure in 12% (v:v), 25, 50 and 100% rapid freezing solution for 2, 1, 1 min and 30-50 s, respectively, at room temperature, and then plunged into liquid nitrogen. Zygotes were rapidly warmed at a speed of 30,000 degrees C/min and subsequently expelled into a graded series of sucrose solutions (1.0, 0.5, 0.25, 0.12 and 0.06 M) at 2.5 min intervals and in vitro cultured for 5 days. If embryos had developed to blastocysts on the 5th day, they were transferred to the recipients. The rest of the embryos were fixed for evaluation of chromatin. RESULTS: Zygote development, up to the expanded blastocyst stage, after in vitro culture, was 40%, if the integrity rate of pronuclei was high, and 4% if the integrity rate was low (P < 0.05). The pregnancy rate after transfer of the 5-day blastocysts depended on the pronuclear integrity rate: 43% (125 pregnancies aftertransfer of 291 blastocysts) for those with a high integrity, and only 1 in 5 (20%) for the few blastocysts which had shown a pronuclear low integrity rate (P < 0.05). CONCLUSIONS: Integrity rate of pronuclei after cryopreservation of pronuclear zygotes was a predictor of future embryo development and implantation: high integrity rate resulted in high pregnancy rate, while zygotes with low integrity rate of pronuclei after cryopreservation had low developmental potential.
Vladimir Isachenko; Plamen Todorov; Yosif Dimitrov; Evgenia Isachenko
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Publication Detail:
Type:  In Vitro; Journal Article     Date:  2008-01-31
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  23     ISSN:  1460-2350     ISO Abbreviation:  Hum. Reprod.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-03-19     Completed Date:  2008-05-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  819-26     Citation Subset:  IM    
Institute of Biology and Immunology of Reproduction, Sofia, Bulgaria.
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MeSH Terms
Blastocyst / physiology*
Embryo Implantation / physiology*
Embryonic Development*
Zygote / growth & development*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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