Document Detail


Integrity and metabolism of human ileal mucosa in vitro in the Ussing chamber.
MedLine Citation:
PMID:  9492901     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Ussing chamber is increasingly being used for in vitro studies of human intestinal mucosa, but little attention has been paid to the viability of specimens over time. Ninety-one mucosal specimens from the ileum in 19 patients operated on for colonic cancer were studied in regard to intestinal barrier function, metabolism, electrophysiology and histology during 360 min of incubation in Ussing chambers. Steady-state permeability to 51Cr-EDTA was maintained for 120 min. Mucosal ATP and lactate levels were stable for 180 min and transmucosal glucose flux for 240 min. Lactate dehydrogenase leakage was limited within 120 min. Transepithelial potential difference was 9.0 +/- 3.0 mV at the start, and declined slowly throughout 360 min. Light microscopy revealed epithelial lifting from the basal lamina at 90 min. Transmission electron microscopy demonstrated preserved ultrastructure for 120 min. Specimens with a transepithelial potential difference below 6 mV at the start were associated with increased 51Cr-EDTA permeability and lactate dehydrogenase leakage and more pronounced light microscopy changes. All studied parameters pointed to preserved viability if experiments were kept within a period of 90 min after equilibration. The few specimens with early viability derangement were identified by a transepithelial potential difference below 6 mV at the start. The Ussing chamber provides a tool for in vitro studies of human intestinal epithelium, including permeability. To minimize viability problems, experiments should be limited in time and monitored by measurements of transepithelial potential difference.
Authors:
J D Söderholm; L Hedman; P Artursson; L Franzén; J Larsson; N Pantzar; J Permert; G Olaison
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta physiologica Scandinavica     Volume:  162     ISSN:  0001-6772     ISO Abbreviation:  Acta Physiol. Scand.     Publication Date:  1998 Jan 
Date Detail:
Created Date:  1998-03-30     Completed Date:  1998-03-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370362     Medline TA:  Acta Physiol Scand     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  47-56     Citation Subset:  IM    
Affiliation:
Department of Surgery and Clinical Research Center, University Hospital, Linköping, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism
Adult
Aged
Aged, 80 and over
Diffusion Chambers, Culture
Female
Glucose / metabolism
Humans
Ileum / enzymology,  metabolism*,  ultrastructure
Intestinal Absorption / physiology*
Intestinal Mucosa / enzymology,  metabolism*,  ultrastructure
L-Lactate Dehydrogenase / metabolism
Lactic Acid / metabolism
Male
Membrane Potentials
Microscopy, Electron
Middle Aged
Permeability
Tissue Survival
Chemical
Reg. No./Substance:
50-21-5/Lactic Acid; 50-99-7/Glucose; 56-65-5/Adenosine Triphosphate; EC 1.1.1.27/L-Lactate Dehydrogenase

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