Document Detail

Integrin-linked kinase complexes with caveolin-1 in human neuroblastoma cells.
MedLine Citation:
PMID:  15654749     Owner:  NLM     Status:  MEDLINE    
Integrin-linked kinase (ILK) and caveolin-1 (cav-1) are implicated in the pathogenesis of cancer. Overexpression of ILK leads to altered expression of cell cycle regulators, a decreased level of cell adhesion to the extracellular matrix, a decreased level of apoptosis, in vitro phosphorylation of Akt, and tumor formation in nude mice. Conversely, cav-1 expression is frequently downregulated in many forms of cancer. We examined whether ILK and cav-1 interact in SHEP human neuroblastoma cells because ILK is present in caveolae-enriched membranes and contains a putative cav-binding domain. SHEP cells were stably transfected with vector, wild-type ILK (ILK-wt), kinase-deficient ILK (ILK-kd), or mutant cav-binding domain ILK (ILK-mutCavbd). Control SHEP cells and ILK transfectants express high levels of ILK and cav-1. Immunoprecipitation with anti-cav-1 co-immunoprecipitates a 59 kDa protein that is immunoreactive with the anti-ILK antibody, and this interaction is partially prevented in cells expressing ILK-mutCavbd. Cav-1 and ILK partially colocalize in SHEP cells, also supporting these data. Last, affinity chromatography with a biotinylated cav-scaffolding domain peptide precipitates ILK-wt but not ILK-mutCavbd. These data suggest that the cav-binding domain of ILK and the cav-scaffolding domain of cav-1 mediate complex formation in human neuroblastoma cells.
Amy Meyer; Cynthia M van Golen; Madanamohan Boyanapalli; Bhumsoo Kim; Mary E Soules; Eva L Feldman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemistry     Volume:  44     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2005-01-18     Completed Date:  2005-04-12     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  932-8     Citation Subset:  IM    
Department of Neurology, University of Michigan, Ann Arbor, Michigan 48109, USA.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Caveolin 1
Caveolins / metabolism*
Cell Line, Tumor
DNA Primers
Fluorescent Antibody Technique
Molecular Sequence Data
Mutagenesis, Site-Directed
Protein-Serine-Threonine Kinases / genetics,  metabolism*
Grant Support
Reg. No./Substance:
0/CAV1 protein, human; 0/Caveolin 1; 0/Caveolins; 0/DNA Primers; EC 2.7.1.-/integrin-linked kinase; EC Kinases

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