Document Detail


Integrin-like proteins are localized to plasma membrane fractions, not plastids, in Arabidopsis.
MedLine Citation:
PMID:  10202813     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Integrins are a large family of integral membrane proteins that function in signal transduction in animal systems. These proteins are conserved in vertebrates, invertebrates, and fungi. Evidence from previous research suggests that integrin-like proteins may be present in plants as well, and that these proteins may function in signal transduction during gravitropism. In past studies, researchers have used monoclonal and polyclonal antibodies to localize beta 1 integrin-like proteins in plants. However, there is a disparity between data collected from these studies, especially since molecular weights obtained from these investigations range from 55-120 kDa for integrin-like proteins. To date, a complete investigation which employs all three basic immunolabeling procedures, immunoblotting, immunofluorescence microscopy, and immunogold labeling, in addition to extensive fractionation and exhaustive controls, has been lacking. In this paper, we demonstrate that use of a polyclonal antibody against the cytoplasmic domain of avian beta 1-integrin can produce potential artifacts in immunolocalization studies. However, these problems can be eliminated through use of starchless mutants or proper specimen preparation prior to electrophoresis. We also show that this antibody, when applied within the described parameters and with careful controls, identifies a large (100 kDa) integrin-like protein that is localized to plasma membrane fractions in Arabidopsis.
Authors:
L J Swatzell; R E Edelmann; C A Makaroff; J Z Kiss
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Plant & cell physiology     Volume:  40     ISSN:  0032-0781     ISO Abbreviation:  Plant Cell Physiol.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-06-07     Completed Date:  1999-06-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9430925     Medline TA:  Plant Cell Physiol     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  173-83     Citation Subset:  IM; S    
Affiliation:
Department of Botany, Miami University, Oxford, OH 45056, USA.
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / chemistry
Cell Fractionation
Cell Membrane / chemistry
Fluorescent Antibody Technique
Immunoblotting
Integrins / analysis*
Microscopy, Fluorescence
Plant Proteins / analysis*
Chemical
Reg. No./Substance:
0/Integrins; 0/Plant Proteins
Investigator
Investigator/Affiliation:
L J Swatzell / Miami U, Oxford, OH; J Z Kiss / Miami U, Oxford, OH

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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