Document Detail

Integrin- and cadherin-mediated induction of the matrix metalloprotease matrilysin in cocultures of malignant oral squamous cell carcinoma cells and dermal fibroblasts.
MedLine Citation:
PMID:  11640889     Owner:  NLM     Status:  MEDLINE    
Matrilysin is a matrix metalloprotease (MMP) overexpressed in a number of cancers including skin, head and neck squamous cell carcinomas, and prostate and colon adenocarcinomas. Matrilysin has been shown to play a role in the degradation of the basement membrane that separates epithelium from stroma allowing tumor cells to intravasate into the bloodstream and metastasize. Here, we show that an oral squamous cell carcinoma cell line (SCC-25) expresses low levels of promatrilysin when cultured alone. However, when SCC-25 cells are cocultured with human foreskin fibroblasts (HFF), there is a 40-fold induction of promatrilysin expression. We tested whether this induction of promatrilysin expression was due to the release of paracrine factors, cell-cell interactions, or cell-matrix interactions. Our results indicate induced promatrilysin expression is the result of both cell-cell and cell-matrix interactions. We demonstrate that beta1 integrins as well as cadherins, specifically N-cadherin and E-cadherin, are involved in the induction of promatrilysin expression. Our results are of general interest in relation to the regulation of MMP expression through cell surface receptor regulation. Further investigation may lead to the identification of novel targets for suppression of invasion and metastasis in oral tumors.
E L Bair; C P Massey; N L Tran; A H Borchers; R L Heimark; A E Cress; G T Bowden
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  270     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-10-19     Completed Date:  2001-12-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  259-67     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Graduate Program, University of Arizona, Tucson, Arizona 85724, USA.
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MeSH Terms
Antigens, CD29 / metabolism*
Blotting, Western
Cadherins / analysis,  genetics,  metabolism*
Calcium / metabolism
Carcinoma, Squamous Cell*
Cell Communication / physiology
Chelating Agents / pharmacology
Coculture Techniques
Dermis / cytology*
Egtazic Acid / pharmacology
Enzyme Precursors / genetics
Extracellular Matrix / physiology
Fibroblasts / cytology,  secretion
Gene Expression Regulation, Enzymologic / drug effects,  physiology
Gene Expression Regulation, Neoplastic / drug effects,  physiology
Matrix Metalloproteinase 7 / genetics*
Metalloendopeptidases / genetics
Mouth Neoplasms*
Peptides / pharmacology
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/Antigens, CD29; 0/Cadherins; 0/Chelating Agents; 0/Enzyme Precursors; 0/Peptides; 67-42-5/Egtazic Acid; 7440-70-2/Calcium; EC 3.4.24.-/Metalloendopeptidases; EC 3.4.24.-/promatrilysin; EC Metalloproteinase 7

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